Tracing residual recombinant feed molecules during digestion and rumen bacterial diversity in cattle fed transgene maize

By R. Einspanier, B. Lutz, S. Rief, O. Berezina, V. Zverlov, W. Schwarz, Johann Mayer. 2004

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Genetically modified soya bean in rabbit feeding: detection of DNA fragments and evaluation of metabolic effects by enzymatic analysis (2006, background): “The persistence of short DNA sequences from plant tissues offered has been shown in the GIT of ruminants, from the oral cavity of sheep to rumen and abomasum ingesta of cattle, differing in the case of maize silage and grain (Duggan et al., 2003; Einspanier et al., 2004).”
A three-year longitudinal study on the effects of a diet containing genetically modified Bt176 maize on the health status and performance of sheep (2008, background): “Thelackofdifferencesobserved in protozoal and bacterial populations is in agreement with results indicated in previous studies ( Einspanier et al., 2004 ).”
A review of the detection and fate of novel plant molecules derived from biotechnology in livestock production (2007, methodology): “Although validation has not yet been reported for complex feed diets, RT-PCR has been applied to silage (Einspanier et al., 2004), commercial feed (Novelli et al., 2003) and a pelleted mixed diet (Alexander et al., 2004).”