Size-distribution analysis of proteins by analytical ultracentrifugation: strategies and application to model systems.

By Peter Schuck, Matthew A. Perugini, Noreen R. Gonzales, G. Howlett, D. Schubert. Biophysical Journal, 2002

677 citations recorded by the source corpus.

Subfields: Biomedical Engineering, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/2797e2e5c05dce8f0e35fe410aba9a52cab0fea9

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In Biomedical Engineering

Peter Schuck

In Molecular Biology

Citations in the corpus

Protein aggregation: pathways, induction factors and analysis. (2009, methodology): “The use of AUC-SV is mainly due to the adoption of the advanced data analysis tools available pioneered by Schuck et al.(149) where the data analysis is based on the continuous sedimentation coefficient distribution method.”
Modern analytical ultracentrifugation in protein science: A tutorial review (2002, methodology): “noise structure of interference optical data has been described ( Schuck et al. 2002 ).”
Characterizing biological products and assessing comparability following manufacturing changes (2004, background): “Part of the resurgence of AU can be attributed to the development of software to deconvolute complicated size distribution”
Challenges in the development of high protein concentration formulations. (2004, background): “protein aggregates and sedimentation coefficients (Schuck 1998; Schuck et al. 2002 ).”
Sedimentation equilibrium analysis of protein interactions with global implicit mass conservation constraints and systematic noise decomposition. (2004, methodology): “of ∆c/∆ω, a situation analogous to time-difference data in sedimentation velocity (41).”
Optimization of the preparation process for human serum albumin (HSA) nanoparticles. (2003, methodology): “In parallel, in part of the samples the size distribution was studied by sedimentation velocity analysis in the analytical ultracentrifuge (Vogel et al., 2002), making use of the superior resolving power of this technique (Schuck, 2000; Schuck and Rossmanith, 2000; Schuck et al., 2002).”
On the analysis of protein self-association by sedimentation velocity analytical ultracentrifugation. (2003, methodology): “In order to rebuild the degree of freedom from the differencing of pair-wise scans in dcdt, the sedimentation model can be combined with systematic noise calculation as described in (52, 68).”