High-efficiency yeast transformation using the LiAc/SS carrier DNA/PEG method

By R. Gietz, R. Schiestl. Nature Protocols, 2007

2,582 citations recorded by the source corpus.

View Paper: https://www.semanticscholar.org/paper/277debccdef6bfa9259851cc5f4dcc5ba0a7a20a

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In Molecular Biology

Citations in the corpus

Rapid generation of potent antibodies by autonomous hypermutation in yeast (2020, methodology): “The ScaI linearized pAW24 or pAW40 plasmids containing desired nanobodies were then transformed into yAW301 by a standard yeast chemical transformation protocol (31).”
Loss of constitutive activity is correlated with increased thermostability of the human adenosine A2A receptor (2013, methodology): “All the A2AR constructs were fusions with a C-terminal GFP8His tag in the p306GPD vector, transformed using the lithium-acetate procedure (Gietz and Schiestl, 2007) and chromosomally integrated at the ura3 locus in the MMY24 (MATa fus1::FUS1-HIS3 LEU2::FUS1-lacZ far1 sst2 ste2 gpa1::ADE2 his3 ura3…”
Deep mutational scanning of SARS-CoV-2 receptor binding domain reveals constraints on folding and ACE2 binding (2020, positive): “Plasmid pools were transformed into the AWY101 strain of Saccharomyces cerevisiae via the protocol of Gietz and Schiestl (Gietz and Schiestl, 2007).”
Metabolic engineering of Saccharomyces cerevisiae for production of fatty acid ethyl esters, an advanced biofuel, by eliminating non-essential fatty acid utilization pathways (2014, methodology): “Yeast transformations for the gene knock-outs construction were performed with a lithium acetate/single-stranded carrier DNA/polyethylene glycol method [35] following a slight modification of cultivation for 6 h under non-selective conditions before plating on antibiotic containing plates, while tra”
Measuring the activity of protein variants on a large scale using deep mutational scanning (2014, methodology): “(iv) Use the culture grown in Step 3B(iii) to prepare competent yeast cells using the high-efficiency LiAc protocol 37 .”
A saturation mutagenesis approach to understanding PTEN lipid phosphatase activity and genotype-phenotypes relationships (2018, methodology): “cerevisiae strain YPH-499, which already contained YCpLG-p110a-CAAX, using the Li-Ac/SS carrier DNA/PEG method.(35) More than 50,000 colony forming units were generated per reaction.”
A versatile toolkit for high throughput functional genomics with Trichoderma reesei (2012, methodology): “Yeast transformation and preparation was performed essentially as described previously [30,49,50].”