Transformation of the diatom Phaeodactylum tricornutum (Bacillariophyceae) with a variety of selectable marker and reporter genes

By L. Zaslavskaia, J. C. Lippmeier, P. Kroth, A. Grossman, K. Apt. 2000

383 citations recorded by the source corpus.

Subfields: Biomaterials, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/246ad4db6a13f17ef455b9d3ab3974d550ec89ef

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Citations in the corpus

Xanthophylls in Microalgae: From Biosynthesis to Biotechnological Mass Production and Application (2003, methodology): “Recently developed transformation techniques for Chlorella [18] and diatoms [2, 26, 90] have potential use in direct commercial applications.”
Genetic Engineering of Algae for Enhanced Biofuel Production (2010, methodology): “tricornutum (2, 210, 211), Navicula saprophila (45), Cylindrotheca fusiformis (52, 148), Cyclotella cryptica (45), and Thalassiosira weissflogii (51); and phaeophytes, such as Laminaria japonica (150) and Undaria pinnatifada (151).”
SILICON METABOLISM IN DIATOMS: IMPLICATIONS FOR GROWTH (2000, background): “…et al. 1997, 1998) and the ability to reintroduce genes into diatoms by transformation (Dunahay et al. 1995, Apt et al. 1996, Fischer et al. 1999, Zaslavskaia et al. 2000), investigations of the molecular details of silicic acid uptake, including determination of individual kinetic parameters…”
Triacylglycerol accumulation and profiling in the model diatoms Thalassiosira pseudonana and Phaeodactylum tricornutum (Baccilariophyceae) during starvation (2009, background): “This information, together with the recent genome sequences of Thalassiosira pseudonana (Hustedt) Hasle and Heimdal clone 3H (Armbrust et al. 2004 )a ndPhaeodactylum tricornutum Bohlin clone CCAP1055/1 and significant advances in the development of molecular genetic tools for the manipulation of the”
MOLECULAR GENETIC MANIPULATION OF THE DIATOM THALASSIOSIRA PSEUDONANA (BACILLARIOPHYCEAE) 1 (2006, methodology): “…Streptomyces noursei (Krügel et al. 1993) (GenBank accession number S60706) for resistance to the antibiotic nourseothricin was amplified from pNAT (Zaslavskaia et al. 2000) by PCR using the sense primer 5 0-ACC AAA TGA CCA CTC TTG ACG ACA C-3 0 and the antisense primer 5 0-GAA TGC GGC CGC CTA…”
Algal transgenics and biotechnology (2007, background): “1996), and the neomycin phosphotransferase II (nptII) gene confers resistance to the aminoglycoside antibiotic G418 (Zaslavskaia et al. 2000).”
ALGAL TRANSGENICS IN THE GENOMIC ERA 1 (2005, background): “The nat1 gene has already been developed as a marker for diatom transformation (Zaslavskaia et al. 2000) and”
Genetic and phenotypic characterization of Phaeodactylum tricornutum (Bacillariophyceae) accessions 1 (2007, methodology): “Genetic transformation of P. tricornutum has only been reported previously for the fusiform morphotype using the English accession Pt1 (Falciatore et al. 1999, Siaut et al. 2007) and the Finnish accession Pt4 (Apt et al. 1996, Zaslavskaia et al. 2000, Kilian and Kroth 2005).”