Small Molecule-Triggered Cas9 Protein with Improved Genome-Editing Specificity

By K. Davis, V. Pattanayak, David B. Thompson, John A. Zuris, David R. Liu. 2015

392 citations recorded by the source corpus.

Directly modulating the activity of genome-editing proteins has the potential to increase their specificity by reducing activity following target locus modification. We developed Cas9 nucleases that are activated by the presence of a cell-permeable small molecule by inserting an evolved 4-hydroxytamoxifen (4-HT)-responsive intein at specific positions in Cas9. In human cells, conditionally active Cas9s modify target genomic sites with up to 25-fold higher specificity than wild-type Cas9.

Subfields: Cardiology and Cardiovascular Medicine, Molecular Biology

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Cardiology and Cardiovascular Medicine

1. Haifeng Wang
2. Lei S. Qi
3. Marie La Russa

In Molecular Biology

1. Haifeng Wang
2. Marie La Russa
3. Lei S. Qi

Citations in the corpus

• Biology and Applications of CRISPR Systems: Harnessing Nature's Toolbox for Genome Engineering. (2016, neutral): “Recent work has focused on developing various chemical- and light-inducible Cas9 constructs to allow for greater spatiotemporal control and on employing Cas9 orthologs with different PAM sequences and smaller sizes, allowing for easier packaging in adeno-associated virus vectors (Davis et al., 2015;”
• CRISPR/Cas9 in Genome Editing and Beyond. (2016, positive): “(158) created intein-Cas9 fusion proteins that produce active Cas9s when a cell-permeable ligand is present.”