Heritable gene targeting in the mouse and rat using a CRISPR-Cas system

By Dali Li, Zhongwei Qiu, Yanjiao Shao, Yuting Chen, Y. Guan, Meizhen Liu, Yongmei Li, Na Gao, Liren Wang, Xiaoling Lu and 2 more. Nature Biotechnology, 2013

658 citations recorded by the source corpus.

Subfields: Genetics, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/2363318118ff83b7333da6f1bbd205888f047329

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In Molecular Biology

1. Jin-Soo Kim
2. Sojung Kim
3. S. Cho
4. Daesik Kim
5. Jungeun Kim
6. Jennifer A. Doudna
7. Lei S. Qi

Citations in the corpus

• Highly efficient RNA-guided genome editing in human cells via delivery of purified Cas9 ribonucleoproteins (2014, methodology): “…to modify genomes in various species including model organisms (Cho et al. 2013b; Dickinson et al. 2013; Friedland et al. 2013; Gratz et al. 2013; Li et al. 2013a,c; Wang et al. 2013; Sung et al. 2014) and plants (Li et al. 2013b; Nekrasov et al. 2013; Shan et al. 2013), rapidly catching up with…”
• Engineering the Delivery System for CRISPR-based Genome Editing (2018, background): “Microinjections can either deliver the cargo into the cytoplasm, or in some cases into the pronucleus of fertilized eggs [41].”
• A CRISPR/Cas9 toolkit for multiplex genome editing in plants (2014, methodology): “More importantly, using this RNA-guided endonuclease technology, multiple gene mutations and their germline transmission have been achieved [28-30].”
• CRISPR/Cas9 systems have off-target activity with insertions or deletions between target DNA and guide RNA sequences (2014, neutral): “In contrast, ”-1 nt” variants of R-30 induced variable cleavage activity at more positions throughout the guide sequence: positions 1, 2 or 3, 7, 8, 9 or 10, 11, 16, 17, 18 and 19 from the PAM (Figure 2D).”
• CRISPR/Cas9-mediated gene editing in human tripronuclear zygotes (2015, positive): “…2013; Cong et al., 2013; Friedland et al., 2013; Hsu et al., 2014; Hwang et al., 2013; Ikmi et al., 2014; Irion et al., 2014; Jinek et al., 2013; Li et al., 2013a; Li et al., 2013b; Long et al., 2014; Ma et al., 2014; Mali et al., 2013c; Niu et al., 2014; Smith et al., 2014a; Wu et al., 2013;…”
• Egg cell-specific promoter-controlled CRISPR/Cas9 efficiently generates homozygous mutants for multiple target genes in Arabidopsis in a single generation (2015, background): “In vertebrates, coinjection of in vitro transcribed Cas9 mRNA and sgRNA into single-cell embryos can produce multi-gene, biallelic mutant animals with high efficiency; the multiple mutations can also be efficiently transmitted to the next generation [11–16].”