The effect of resting condition on resting-state fMRI reliability and consistency: A comparison between resting with eyes open, closed, and fixated

By Rémi Patriat, Erin K. Molloy, Timothy B. Meier, Gregory R. Kirk, Veena A. Nair, Mary Elizabeth Meyerand, Vivek Prabhakaran, Rasmus M. Birn. NeuroImage, 2013

387 citations recorded by the source corpus.

Resting-state fMRI (rs-fMRI) has been demonstrated to have moderate to high reliability and produces consistent patterns of connectivity across a wide variety of subjects, sites, and scanners. However, there is no one agreed upon method to acquire rs-fMRI data. Some sites instruct their subjects, or patients, to lie still with their eyes closed, while other sites instruct their subjects to keep their eyes open or even fixating on a cross during scanning. Several studies have compared those three resting conditions based on connectivity strength. In our study, we assess differences in metrics of test–retest reliability (using an intraclass correlation coefficient), and consistency of the rank-order of connections within a subject and the ranks of subjects for a particular connection from one session to another (using Kendall's W tests). Twenty-five healthy subjects were scanned at three different time points for each resting condition, twice the same day and another time two to three months later. Resting-state functional connectivity measures were evaluated in motor, visual, auditory, attention, and default-mode networks, and compared between the different resting conditions. Of the networks examined, only the auditory network resulted in significantly higher connectivity in the eyes closed condition compared to the other two conditions. No significant between-condition differences in connectivity strength were found in default mode, attention, visual, and motor networks. Overall, the differences in reliability and consistency between different resting conditions were relatively small in effect size but results were found to be significant. Across all within-network connections, and within default-mode, attention, and auditory networks statistically significant greater reliability was found when the subjects were lying with their eyes fixated on a cross. In contrast, primary visual network connectivity was most reliable when subjects had their eyes open (and not fixating on a cross).

Subfields: Cognitive Neuroscience, Radiology, Nuclear Medicine and Imaging

View Paper: https://www.semanticscholar.org/paper/231360f3bcdcc1674861d108fd6a831ba2cdc13f

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In Cognitive Neuroscience

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Citations in the corpus

The effect of scan length on the reliability of resting-state fMRI connectivity estimates (2013, neutral): “While differences in connectivity strength and reliability across resting conditionsmay be statistically significant for some networks, the differences are relatively small (Patriat et al., 2013).”
ICA-based artifact removal diminishes scan site differences in multi-center resting-state fMRI (2015, background): “…other studies Frontiers in Neuroscience | www.frontiersin.org 7 October 2015 | Volume 9 | Article 395 showed higher BOLD response (McAvoy et al., 2008) and higher mean ALFF (Jao et al., 2013) for the eyes-closed condition, or no difference in seed-based correlations (Patriat et al., 2013).”
Building a Science of Individual Differences from fMRI. (2016, background): “Of course, careful investigations have pointed out ‘details’ that influence the data collected during the resting-state, such as whether the subject's eyes are open or closed [161], whether they are completely awake [124], what they are actually thinking about during the run [162–164], or what task ”