Inactivation of the coronavirus that induces severe acute respiratory syndrome, SARS-CoV
By M. Darnell, K. Subbarao, S. Feinstone, Deborah R. Taylor. Journal of Virological Methods, 2004
664 citations recorded by the source corpus.
Severe acute respiratory syndrome (SARS) is a life-threatening disease caused by a novel coronavirus termed SARS-CoV. Due to the severity of this disease, the World Health Organization (WHO) recommends that manipulation of active viral cultures of SARS-CoV be performed in containment laboratories at biosafety level 3 (BSL3). The virus was inactivated by ultraviolet light (UV) at 254 nm, heat treatment of 65 °C or greater, alkaline (pH > 12) or acidic (pH < 3) conditions, formalin and glutaraldehyde treatments. We describe the kinetics of these efficient viral inactivation methods, which will allow research with SARS-CoV containing materials, that are rendered non-infectious, to be conducted at reduced safety levels.
Subfields: Infectious Diseases, Pulmonary and Respiratory Medicine
View Paper: https://www.semanticscholar.org/paper/21fcb7a2c9477d5a564bd7c2f4fcae993bf590e6
How researchers see this paper
The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.
In Infectious Diseases
Citations in the corpus
- The Effects of Temperature and Relative Humidity on the Viability of the SARS Coronavirus (2011, background): “Another study showed that it was inactivated by ultraviolet light, alkaline (pH> 12), or acidic (pH< 3) conditions [11].”
- SARS-CoV-2 RNA in wastewater anticipated COVID-19 occurrence in a low prevalence area (2020, background): “As well, the elevated sensitivity of human pathogenic coronaviruses to environmental conditions (Chin et al., 2020; Darnell et al., 2004; Darnell and Taylor, 2006) and disinfectants (Chin et al., 2020; J. Wang et al., 2020a) suggests a poor risk of transmission via wastewater, even though formal…”