A new pathway for synthesis of phosphatidylinositol-4,5-bisphosphate

By L. Rameh, K. Tolias, B. Duckworth, Lewis C. Cantley. 1997

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Citations in the corpus

• PIP4Ks Suppress Insulin Signaling through a Catalytic-Independent Mechanism (2018, unknown): “PIP5K1A, PIP5K1B, and PIP5K1C produce PI(4,5)P 2 from phosphati-dylinositol-4-phosphate (PI(4)P), while PIP4K2A, PIP4K2B, and PIP4K2C generate PI(4,5)P 2 from phosphatidylinositol-5-phos-phate (PI(5)P) (Rameh et al., 1997; van den Bout and Divecha, 2009).”
• Inhibition of PIP4Kγ ameliorates the pathological effects of mutant huntingtin protein (2017, background): “The protein is predominantly localized in several tissues, including the brain (Sasaki et al., 2009; Rameh et al., 1997; Clarke et al., 2008; Clarke et al., 2009).”
• PI5P4Kγ functions in DTX1-mediated Notch signaling (2018, background): “PIP5K2C encodes a lipid kinase (PI5P4Kγ) that phosphorylates PI5P to generate PI(4,5)P2 (37).”
• Increased Insulin Sensitivity and Reduced Adiposity in Phosphatidylinositol 5-Phosphate 4-Kinase β−/− Mice (2004, background): “Mammals have three isoforms of PI-5-P 4-kinase (PI5P4K , PI5P4K , and PI5P4K ) encoded by distinct genes (9, 12, 17), and it was recently shown that overexpression of any of these three enzymes diminishes the insulin-stimulated activation of Akt by reducing the amount of PI-3,4,5-P3 produced after i”
• Phosphatidylinositol 5-phosphate 4-kinase (PIP4K) regulates TOR signaling and cell growth during Drosophila development (2013, background): “S1) and this substrate specificity recapitulates that of human PIP4Kα under the same conditions (8, 21).”
• Depletion of a Putatively Druggable Class of Phosphatidylinositol Kinases Inhibits Growth of p53-Null Tumors (2013, background): “…family of PIP kinases (called type 2) was found to produce PI-4,5-P2 by phosphorylating the 4 position of phosphatidylinositol-5-phosphate (PI-5-P), a lipid that had been previously overlooked due to its comigration with the much more abundant PI-4-P (Rameh and Cantley, 1999; Rameh et al., 1997).”
• A Homogeneous, High-Throughput Assay for Phosphatidylinositol 5-Phosphate 4-Kinase with a Novel, Rapid Substrate Preparation (2013, methodology): “Additionally, lowthroughput radiolabeled enzymatic thin layer chromatography (TLC) assays were developed where the substrate was prepared in an isotonic KCl solution or as a liposome mixture [20,21].”
• Phosphoinositides: tiny lipids with giant impact on cell regulation. (2013, background): “1020 Physiol Rev • VOL 93 • JULY 2013 • www.prv.org by 10.220.33.3 on O ctober 27, 2017 http://physrev.physiology.org/ D ow nloaded from HO HO OH OH OH 12 3 4 5 6OH 1 2 3 4 5 6 myo-inositolAgranoff’s turtle PtdIns A B P O O O HO HO OH OH OH 12 3 4 5 6 O Stearoyl Arachidonyl PtdIns 8 5 8 8 9 10 12 13”
• PI(5)P Regulates Autophagosome Biogenesis (2015, background): “, 2012), is PI(4)P in resting cells (Rameh et al., 1997; Whiteford et al., 1997).”
• Phosphatidylinositol-5-phosphate 4-kinases regulate cellular lipid metabolism by facilitating autophagy (2018, background): “The phosphatidylinositol-5-phosphate 4-kinase (PI5P4K) family of enzymes provides an alternative pathway for generating PI-4,5-P2 at intracellular locations by phosphorylating phosphatidylinositol 5-monophosphate (PI-5-P) (Rameh et al., 1997).”