[16] Purification of proteins using polyhistidine affinity tags

By J. Bornhorst, J. Falke. 2000

343 citations recorded by the source corpus.

Subfields: Ecology, Molecular Biology, Radiology, Nuclear Medicine and Imaging

View Paper: https://www.semanticscholar.org/paper/205eeb02f2a7519c2a7ac37ad579407ed8b8acd0

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Ecology

1. E. Ceccarelli
2. Germán L. Rosano

In Molecular Biology

1. E. Ceccarelli
2. Germán L. Rosano

In Radiology, Nuclear Medicine and Imaging

1. E. Ceccarelli
2. Germán L. Rosano

Citations in the corpus

• Recombinant protein expression in Escherichia coli: advances and challenges (2014, methodology): “…can be recovered by immobilized metal ion affinity chromatography using Ni2+ or Co2+-loaded nitrilotriacetic acid-agarose resins (Porath and Olin, 1983; Bornhorst and Falke, 2000), while anti-FLAG affinity gels (Sigma-Aldrich) are used for capturing FLAG fusion proteins (Hopp et al., 1988).”