Comparing protein abundance and mRNA expression levels on a genomic scale

By D. Greenbaum, C. Colangelo, Kenneth R. Williams, Mark Gerstein. Genome Biology, 2003

1,780 citations recorded by the source corpus.

Attempts to correlate protein abundance with mRNA expression levels have had variable success. We review the results of these comparisons, focusing on yeast. In the process, we survey experimental techniques for determining protein abundance, principally two-dimensional gel electrophoresis and mass-spectrometry. We also merge many of the available yeast protein-abundance datasets, using the resulting larger 'meta-dataset' to find correlations between protein and mRNA expression, both globally and within smaller categories.

Subfields: Molecular Biology, Spectroscopy

View Paper: https://www.semanticscholar.org/paper/1e39d068cf506a4be8adc271b177e836ac6922b1

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Molecular Biology

In Spectroscopy

Citations in the corpus

The mathematics of tanning (2009, neutral): “Genes with low correlation between their transcriptomic and proteomic levels could be substantially regulated by post-transcriptional mechanisms [ 22 ].”
A practical guide to linking brain-wide gene expression and neuroimaging data (2018, background): “…as several studies have shown that mRNA and protein levels within a tissue can vary signi ﬁ - cantly (Futcher et al., 1999; Gygi et al., 1999; Greenbaum et al., 2003), and gene expression (transcriptional activity) and protein abundance (translational activity) are not always positively…”
The Skyline Ecosystem: Informatics for Quantitative Mass Spectrometry Proteomics (2017, background): “…is highlighted by the observation that measurements performed at the nucleic acid level tend to correlate very poorly with those performed at the protein level (Greenbaum et al. 2003; Schrimpf et al. 2009), especially in cases when experimental noise is not considered (Csardi et al. 2015).”
Correlation of mRNA and protein in complex biological samples (2009, neutral): “Greenbaum et al. [41] integrated these and other studies on protein abundance in yeast.”