Identification of arbuscular mycorrhizal fungi in soils and roots of plants colonizing zinc wastes in southern Poland

By K. Turnau, P. Ryszka, V. Gianinazzi‐Pearson, D. V. Tuinen. Mycorrhiza, 2001

154 citations recorded by the source corpus.

Abstract Analysis of the community of arbuscular mycorrhizal (AM) fungi in roots of Fragaria vesca growing in a heavy metal contaminated site was carried out on a Zn waste site near Chrzanow (southern Poland). The waste substratum was characterized by high contents of Pb, Zn, Cd, Cu and As, and by low levels of N, P and organic matter. Spores of Glomales were isolated by wet sieving and DNA was isolated from individual spores. Nested polymerase chain reaction (PCR) with taxon-specific primers was used to identify the species Glomus mosseae, Glomus intraradices and Glomus claroideum. Spores of other fungi were morphologically characterized and new taxon-discriminating molecular probes were developed for two of them (Glomus sp. HM-CL4 and HM-CL5) based on variations in the large ribosomal subunit (25S rDNA). High sequence similarities were found between Glomus sp. HM-CL4 and Glomus gerdemanii, and between Glomus sp. HM-CL5 and Glomus occultum. The designed primers were used to characterize the population of AM fungi colonizing the roots of F. vesca collected from the Zn waste site. The analysis, carried out on roots stained with trypan blue, showed that the most effective colonizer was closely related to G. gerdemannii. G. claroideum and the G. occultum-like fungus were slightly less common whilst frequencies of G. intraradices and G. mosseae in roots were much lower. The analysis of mycorrhiza stained with rhodizoniate to localize heavy metal accumulation showed that the stain does not influence the PCR reaction. Seventy percent of the root samples containing positively stained fungal hyphae were found to be colonized by G. mosseae. The data obtained demonstrate the usefulness of nested PCR for studies carried out in polluted areas. It will enable selection of AM fungi which are able to colonize plant roots under heavy metal stress conditions, as well as the identification of fungi showing high in situ accumulation of potentially toxic elements.

Subfields: Cell Biology, Pharmacology, Plant Science

View Paper: https://www.semanticscholar.org/paper/1b3587af4f51a5dd4e97cc9456c3f84017f38d65

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Citations in the corpus

• Prospects of arbuscular mycorrhizal fungi in phytoremediation of heavy metal contaminated soils (2004, background): “Turnau et al.(52) analysed the community of AM fungi in roots of Fragaria vesca growing in Zn-contaminated soil.”
• Plant-driven selection of microbes (2009, background): “(Hempel et al. 2007; Mathimaran et al. 2005; Pivato et al. 2007; Turnau et al. 2001; Vandenkoornhuyse et al. 2003) The differences in colonization speed and mycelium extension observed between the different arbuscular mycorrhizal fungi emphasizes the dynamics of the mycorrhizosphere in time and…”
• Mycorrhiza : state of the art, genetics and molecular biology, eco-function, biotechnology, eco-physiology, structure and systematics (2008, background): “Different isolates have diverse tolerance to excess levels of many trace elements (Turnau et al. 2001).”
• Heavy metal detoxification and tolerance mechanisms in plants: Implications for phytoremediation (2016, background): “352 Turnau et al. (2001) examined the AM fungi in roots of Fragaria vesca growing in Zn-353 contaminated soil and found that about 70% of the root sample was colonized with G. mosseae.”
• Molecular Mechanism of Heavy Metal Toxicity and Tolerance in Plants: Central Role of Glutathione in Detoxification of Reactive Oxygen Species and Methylglyoxal and in Heavy Metal Chelation (2012, background): “A complete understanding of the molecular bases of HM tolerance in this symbiotic system will, thus, also aid the selection of the most effective AM isolates [367] and plantfungus combinations for bioremediation and soil protection [368].”
• Phytoremediation of Metal-Polluted Soils by Arbuscular Mycorrhizal Fungi (2012, background): “…one of these functions contributes to the protection of the plant against environmental stress, thus promoting soil revegetation in ecosystems that have been degraded by discharge of high metal concentrations (Gaur and Adholeya 2004; Leung et al., 2006; Smith and Read, 1997; Turnau et al., 2001).”
• Species composition of arbuscular mycorrhizal fungi in two mountain meadows with differing management types and levels of plant biodiversity (2006, background): “This species was also found by Turnau et al. (2001) to be one of the dominating fungi at zinc waste sites in Poland, suggesting a high tolerance towards different ecological conditions.”
• SOIL TILLAGE AFFECTS THE COMMUNITY STRUCTURE OF MYCORRHIZAL FUNGI IN MAIZE ROOTS (2003, methodology): “At that time the necessity of tuning the identification tools for local AMF communities (including extensive isolation of AMF from the studied site) had not yet been recognized (Turnau et al. 2001).”
• The contribution of arbuscular mycorrhizal fungi in sustainable maintenance of plant health and soil fertility (2002, background): “This might play an important role in the initial steps of the establishment of wetland places, and subsequently could influence plant biodiversity in later stages, encouraging the re-appearance of mycorrhizal species (Vangronsveld et al. 1996).”
• Community structure of arbuscular mycorrhizal fungi associated with pioneer grass species Miscanthus sinensis in acid sulfate soils: Habitat segregation along pH gradients (2008, methodology): “Recently, this method has been successfully applied to AM fungal community analyses in various ecosystems (e.g. Gollotte et al. 2004; Pivato et al. 2007; Turnau et al. 2001).”