Random GFP::cDNA fusions enable visualization of subcellular structures in cells of Arabidopsis at a high frequency.

By Sean Cutler, D. Ehrhardt, Joel S. Griffitts, Chris Somerville. Proceedings of the National Academy of Sciences of the United States of America, 2000

1,004 citations recorded by the source corpus.

Subfields: Biophysics, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/1a52ca4944b549e54f8ee89672d6eb1682a2ec08

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Citations in the corpus

SCD1 is required for cell cytokinesis and polarized cell expansion in Arabidopsis thaliana (2003, methodology): “Cotyledons of 13-day-old wild-type (A) and scd1-1 seedlings (B,C) that express the nuclear (green) GFP N7 marker and (D) cotyledons from 4-day-old seedlings expressing plasma membrane GFP 29-1 marker ( Cutler et al., 2000 ).”
Rapid, combinatorial analysis of membrane compartments in intact plants with a multicolor marker set. (2009, background): “Earlier attempts to generate such sets had limited success, being restricted to random cDNA fusions that did not allow for the parallel generation of multiple colors (Cutler et al., 2000).”
A multicolored set of in vivo organelle markers for co-localization studies in Arabidopsis and other plants. (2007, background): “FP fusions also have the potential of being scaled up for high-throughput screens (Cutler et al., 2000; Escobar et al., 2003; Koroleva et al., 2005; Tian et al., 2004).”
The FERONIA Receptor Kinase Maintains Cell-Wall Integrity during Salt Stress through Ca2+ Signaling (2018, methodology): “Single cell death events were counted as a loss in LTI6-YFP signal.”
Overexpression of a cytosolic glyceraldehyde-3-phosphate dehydrogenase gene OsGAPC3 confers salt tolerance in rice (2011, methodology): “The cDNA fragments were digested with BamHI and fused in-frame with the coding region of GFP (Green Fluorescence Protein) in the pEGAD vector (Cutler et al. 2000), in which expression is driven by the CaMV 35S promoter.”
Direct regulation of the NADPH oxidase RBOHD by the PRR-associated kinase BIK1 during plant immunity. (2014, background): “In contrast, LTI6b, a plasmamembrane marker (Cutler et al., 2000), did not associate with RBOHD (Figures 1B and S1B).”
Light signal transduction in higher plants. (2004, background): “Direct DNA binding of cry1 and cry2 has not been reported; however, a cry2 carboxy-terminal extension-GFP fusion is chromatin associated (34).”
Arabidopsis PEN3/PDR8, an ATP Binding Cassette Transporter, Contributes to Nonhost Resistance to Inappropriate Pathogens That Enter by Direct Penetration[W][OA] (2006, methodology): “tumefaciens–containing infiltration medium as described (Cutler et al., 2000).”