Semisynthesis of proteins by expressed protein ligation.

By T. Muir. Annual Review of Biochemistry, 2003

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Design of Functional Metalloproteins (2009, positive): “Expressed-protein ligation, which involves the coupling of a bacterially expressed peptide fragment with a synthetic peptide fragment containing unnatural amino acids, has reduced the cost and extended the protein size even further, but it often requires a Cys residue at the point of ligation to max”
Nucleosome-Interacting Proteins Regulated by DNA and Histone Methylation (2010, background): “The methylation of lysines in H3 was accomplished by native chemical ligation (Muir, 2003).”