Carbon nanotubes as intracellular protein transporters: generality and biological functionality.

By N. W. Kam, Hongjie Dai. Journal of the American Chemical Society, 2005

984 citations recorded by the source corpus.

Various proteins adsorb spontaneously on the sidewalls of acid-oxidized single-walled carbon nanotubes. This simple nonspecific binding scheme can be used to afford noncovalent protein-nanotube conjugates. The proteins are found to be readily transported inside various mammalian cells with nanotubes acting as the transporter via the endocytosis pathway. Once released from the endosomes, the internalized protein-nanotube conjugates can enter into the cytoplasm of cells and perform biological functions, evidenced by apoptosis induction by transported cytochrome c. Carbon nanotubes represent a new class of molecular transporters potentially useful for future in vitro and in vivo protein delivery applications.

Subfields: Biomedical Engineering, Cellular and Molecular Neuroscience, Materials Chemistry

View Paper: https://www.semanticscholar.org/paper/17980fbe964eaeedc93022a865346dbf820dc0ca

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In Biomedical Engineering

In Cellular and Molecular Neuroscience

In Materials Chemistry

Citations in the corpus

Cellular uptake of functionalized carbon nanotubes is independent of functional group and cell type. (2007, background): “Other studies indicate that CNTs coated with protein”
Shape effects of filaments versus spherical particles in flow and drug delivery. (2007, methodology): “We verified with filomicelles in whole blood in vitro for one week (at 37 8C) that (1) dye intensity is constant, (2) dye does not transfer to label blood cell membranes, and ( 3 ) filomicelles exhibit a constant length distribution.”
Basics and recent advances in peptide and protein drug delivery (2013, positive): “One example, the carbon nanotube, is well-studied and has been used to deliver proteins [191].”
Beneficial role of carbon nanotubes on mustard plant growth: an agricultural prospect (2011, background): “Investigations are increasingly focusing on carbon nanotubes (CNTs) in contrast to other nanoparticles due to their ability to penetrate mammalian and bacterial cells (Wong et al. 2005; Zhu et al. 2007).”
Biomedical applications of functionalised carbon nanotubes. (2005, background): “These functionalised CNTs are capable of carrying proteins and DNA into cells via the endocytosis pathway (Kam and Dai, 2005; Kam et al., 2006).”
Oxidation and reduction of multiwalled carbon nanotubes — preparation and characterization (2010, background): “[13] Shi KamNW,Dai H.”