A Versatile Synthetic Extracellular Matrix Mimic via Thiol-Norbornene Photopolymerization
By Benjamin D Fairbanks, Michael P. Schwartz, Alexandra E. Halevi, C. Nuttelman, Christopher N. Bowman, Kristi S. Anseth. Advances in Materials, 2009
627 citations recorded by the source corpus.
Synthetic hydrogels with engineered, cell-mediated degradation sites are an important category of biomimetic materials. Here, hydrogels are synthesized by a step-growth reaction mechanism via a radically mediated thiol-norbornene (thiol-ene) photopolymerization. This reaction combines the advantages of ideal, homogeneous polymer network formation, facile incorporation of peptides without post-synthetic modification, and spatial and temporal control over the network evolution into a single system to produce proteolytically degradable poly(ethylene glycol) (PEG) peptide hydrogels. Using a thiol-ene photopolymerization, rapid gelation times are achieved, while maintaining high cell viability for cell encapsulation. The enzyme- and cellresponsive characteristics are demonstrated by tailoring the rate of spreading of human mesenchymal stem cells (hMSCs) through both the selection of proteolytically degradable crosslinkers and the density of the adhesion peptide RGDS. Furthermore, cellular function is manipulated spatially within the thiol-ene hydrogels through biochemical photopatterning. The high degree of spatial and temporal control over gelation, combined with robust material properties, makes thiol-ene hydrogels an excellent tool for a variety of medical and biological applications.
Subfields: Biomaterials, Organic Chemistry, Surfaces, Coatings and Films
View Paper: https://www.semanticscholar.org/paper/175e8b25b026825170f449369b2b98c0f09e5132
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In Surfaces, Coatings and Films
Citations in the corpus
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