Fluoro-Jade B: a high affinity fluorescent marker for the localization of neuronal degeneration.

By L. Schmued, K. Hopkins. Brain Research, 2000

1,243 citations recorded by the source corpus.

Fluoro-Jade B, like its predecessor Fluoro-Jade, is an anionic fluorescein derivative useful for the histological staining of neurons undergoing degeneration. However, Fluoro-Jade B has an even greater specific affinity for degenerating neurons. This notion is supported by the conspicuous staining of degenerating neuronal elements with minimal background staining. This improved signal-to-noise ratio means that fine neuronal processes including distal dendrites, axons and axon terminals can be more readily detected and documented. Although the staining time and dye concentration are reduced, the method is as rapid, simple and reliable as the original Fluoro-Jade technique. Like Fluoro-Jade, Fluoro-Jade B is compatible with a number of other labeling procedures including immunofluorescent and fluorescent Nissl techniques.

Subfields: Cellular and Molecular Neuroscience, Neurology, Physiology

View Paper: https://www.semanticscholar.org/paper/15c879aa390a5f3712088f4776a0fe4ca915f84a

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Citations in the corpus

Tau oligomers impair memory and induce synaptic and mitochondrial dysfunction in wild-type mice (2011, methodology): “Fluoro-Jade B binds to polyaminergic products of cellular degeneration, such as spermidine, cadaverine, and putrescine [38,39].”
Glucagon-like peptide-1 receptor is involved in learning and neuroprotection (2003, methodology): “Immunohistochemical comparison of the CA3 subregion of the hippocampus using Fluorojade-B, a fluorochrome stain specific for degenerating neuron”
Blood-brain barrier leakage may lead to progression of temporal lobe epilepsy. (2007, positive): “To evaluate whether tracer/albumin containing cells were degenerating cells, a Fluoro-Jade B (FJB) staining was performed as described previously (Schmued and Hopkins, 2000) on a subset of sections (at least two sections/rat, 2.4, 3.4 and 4.6 mm lateral to bregma) of rats that were injected with EB.”
Impaired spatial representation in CA1 after lesion of direct input from entorhinal cortex. (2008, methodology): “To map the cell loss with a more sensitive approach, we first assessed neuronal degeneration in four lesioned animals not implanted for recording, making use of a specific marker for neuronal degeneration, Fluoro-Jade B (Schmued and Hopkins, 2000).”