Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances

By I. Wiegand, K. Hilpert, Robert E. W. Hancock. Nature Protocols, 2008

5,755 citations recorded by the source corpus.

Subfields: Food Science, Microbiology, Pollution

View Paper: https://www.semanticscholar.org/paper/159baac7c1baa442660ea28e76be0780b6f9d53b

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Food Science

1. C. Manyi-Loh
2. Anthony I. Okoh
3. M. Černík
4. S. Mamphweli
5. E. Meyer
6. V. Padil

In Microbiology

1. M. Černík
2. V. Padil

In Pollution

1. Roger Marchant
2. C. Manyi-Loh
3. D. Fatta-Kassinos
4. Anthony I. Okoh
5. M. Černík
6. S. Mamphweli
7. I. Banat
8. E. Meyer

Citations in the corpus

• Green synthesis of copper oxide nanoparticles using gum karaya as a biotemplate and their antibacterial application (2013, positive): “aureus MTCC 737 were used as reference strains for Gram-negative and Gram-positive bacteria, respectively, following guidelines of the Clinical and Laboratory Standards Institute.(33) Strains were grown on nutrient agar.”
• Urban wastewater treatment plants as hotspots for antibiotic resistant bacteria and genes spread into the environment: a review. (2013, background): “Antibiotic resistance testing is highly standardized worldwide, enabling laboratories to assist clinicians in bacterial infection therapy (CLSI, 2007; WHO, 2008; Wiegand et al., 2008).”
• Resazurin-based 96-well plate microdilution method for the determination of minimum inhibitory concentration of biosurfactants (2016, positive): “The minimal inhibitory concentration (MIC), which is a key indicator of an antimicrobial agent’s potency, is defined as the concentration (mg l - 1 ) at which visible growth of bacteria is prevented under defined growth conditions (Wiegand et al. 2008).”
• Novel isolates of lactobacilli from fermented Portuguese olive as potential probiotics (2014, methodology): “Screening for antibiotic resistance was via determination of MIC e as the lowest concentration of antimicrobial agent that inhibits naked eye-visible growth (Wiegand et al., 2008).”
• Functional Gold Nanoparticles as Potent Antimicrobial Agents against Multi-Drug-Resistant Bacteria (2014, methodology): “We first evaluated the functional AuNP antimicrobial activities on a laboratory strain (Escherichia coli DH5R), using broth dilution methods to determine the minimal inhibitory concentrations (MICs).(25) AuNPs were incubated with 5 10(5) cfu/mL of E.”
• Cryptic prophages help bacteria cope with adverse environments (2010, background): “Note that MIC values are based on turbidity so they do not discriminate between bactericidal or bacteriostatic actio”
• Describing the Mechanism of Antimicrobial Peptide Action with the Interfacial Activity Model (2010, neutral): “Biological assays are carried out at 104–106 bacterial cells mL 1 (58).”
• Bio compounds of edible mushrooms: in vitro antioxidant and antimicrobial activities (2019, neutral): “Antimicrobial activities of optimized mushroom extracts were tested against 4 bacteria: 2 Gram-positive ( Staphylococcus aureus ATCC Bacterial suspensions were prepared according to the protocol described by Wiegand, Hilpert, and Hancock (2008).”
• Antibiotic Use in Agriculture and Its Consequential Resistance in Environmental Sources: Potential Public Health Implications (2018, background): “It can be determined both by micro broth dilution in microplates [115], and agar dilution [116].”
• The Minimum Inhibitory Concentration of Antibiotics: Methods, Interpretation, Clinical Relevance (2021, background): “This is due to the fact that over time, the medium accumulates oxygen, which in turn reduces the activity of tigecycline [3,4,6,9].”