Protectants used in the cryopreservation of microorganisms.

By Z. Hubálek. Cryobiology, 2003

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Cryopreservation of Phytodiversity: A Critical Appraisal of Theory & Practice (2008, background): “In the case of microorganisms, the empirical study of cryoprotection (Hubálek, 2003) is complemented by studies of coldinduced gene expression (Homma et al., 2003).”
Use of alginate and cryo-protective sugars to improve the viability of lactic acid bacteria after freezing and freeze-drying (2005, background): “To prevent or reduce these adverse effects, many substances are used as cryoprotectives (Carcoba & Rodriguez 2000; Hubalek 2003).”
Preservation of micro-organisms by drying; a review. (2006, background): “These include non-fat milk solids, serum, trehalose, glycerol, betaine, adonitol, sucrose, glucose, lactose and polymers such as dextran and polyethylene glycol (Hubalek, 2003).”
Working with rhizobia (2016, methodology): “Several cryoprotectors have been employed including dimethylsulfoxide, glycerol, albumin, skim milk and peptone (Hubálek 2003; Day and Stacey 2007).”