3D Bioprinting of Heterogeneous Aortic Valve Conduits with Alginate/Gelatin Hydrogels

By B. Duan, L. Hockaday, K. H. Kang, Jonathan T. Butcher. Journal of Biomedical Materials Research. Part A, 2012

924 citations recorded by the source corpus.

Heart valve disease is a serious and growing public health problem for which prosthetic replacement is most commonly indicated. Current prosthetic devices are inadequate for younger adults and growing children. Tissue engineered living aortic valve conduits have potential for remodeling, regeneration, and growth, but fabricating natural anatomical complexity with cellular heterogeneity remain challenging. In the current study, we implement 3D bioprinting to fabricate living alginate/gelatin hydrogel valve conduits with anatomical architecture and direct incorporation of dual cell types in a regionally constrained manner. Encapsulated aortic root sinus smooth muscle cells (SMC) and aortic valve leaflet interstitial cells (VIC) were viable within alginate/gelatin hydrogel discs over 7 days in culture. Acellular 3D printed hydrogels exhibited reduced modulus, ultimate strength, and peak strain reducing slightly over 7-day culture, while the tensile biomechanics of cell-laden hydrogels were maintained. Aortic valve conduits were successfully bioprinted with direct encapsulation of SMC in the valve root and VIC in the leaflets. Both cell types were viable (81.4±3.4% for SMC and 83.2±4.0% for VIC) within 3D printed tissues. Encapsulated SMC expressed elevated alpha-smooth muscle actin when printed in stiff matrix, while VIC expressed elevated vimentin in soft matrix. These results demonstrate that anatomically complex, heterogeneously encapsulated aortic valve hydrogel conduits can be fabricated with 3D bioprinting.

Subfields: Biomaterials, Biomedical Engineering, Surgery

View Paper: https://www.semanticscholar.org/paper/125b685aa18125bcfb27d57d9916549e3b76f7fa

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Biomaterials

In Biomedical Engineering

In Surgery

Citations in the corpus

A comprehensive review on droplet-based bioprinting: Past, present and future. (2016, background): “root smooth muscle cells 84% ~ Ф3 cm 3 cm tall constructs [162]”
Potential Development of Sustainable 3D-Printed Meat Analogues: A Review (2021, neutral): “, agarose, alginate, gelatin, chitosan) [44] may be used for the 3DP meat analog.”
3D Bioprinting for Engineering Complex Tissues (2015, background): “Arti ﬁ - cial tissues are seeded by either printing functional primary cells with supporting cells (Keriquel et al., 2010; Cui et al., 2012a; Duan et al., 2013; Michael et al., 2013; Xu et al., 2013; Zhang et al., 2013; Dolati et al., 2014) or printing progenitors or stem cells for further…”