A complementary transposon tool kit for Drosophila melanogaster using P and piggyBac

By S. Thibault, M. Singer, W. Y. Miyazaki, B. Milash, Nicholas A Dompe, Carol M Singh, R. Buchholz, Madelyn Demsky, R. Fawcett, H. Francis-Lang and 27 more. Nature Genetics, 2004

900 citations recorded by the source corpus.

Subfields: Immunology, Insect Science, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/122a3c23bec97dbc630d5e7dddd93539db328907

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Citations in the corpus

Uncovering the essential genome of the human malaria parasite Plasmodium falciparum by saturation mutagenesis (2018, positive): “Transposon mutagenesis using the piggyBac transposon, which preferentially inserts at the tetranucleotide target sequence TTAA, has been used to carry out whole-genome loss-of-function screens in many organisms (8-11).”
A program for annotating and predicting the effects of single nucleotide polymorphisms, SnpEff (2012, positive): “, mitochondrial genomes); (5) integration with Broad's Genome Analysis Toolkit (GATK);(4) and (6) ability to perform non-coding annotations.”
D1 Dopamine Receptor dDA1 Is Required in the Mushroom Body Neurons for Aversive and Appetitive Learning in Drosophila (2007, background): “When MB247-GAL4/UAS-GFP in the wild-type background was stained with the dDA1 antibody, the GFP-labeled (thus MB247-GAL4-expressing) MB neurons were positive for dDA1 IRs although the relative intensities of GFP and dDA1 signals varied in the different MB lobes (Fig.”
γ neurons mediate dopaminergic input during aversive olfactory memory formation in Drosophila (2012, neutral): “piggyBac (WH) carries a UAS enhancer for Gal4-driven expression of the flanking adjacent gene [14].”
Genetic manipulation of genes and cells in the nervous system of the fruit fly (2011, background): “They exhibit a strong insertional bias for promoters and origin of replications binding sites (Bellen et al., 2011). piggyBac transposons do not show a strong insertional bias, but mobilize less efficiently than P elements, and only excise precisely (Thibault et al., 2004; Witsell et al., 2009).”
Normal microRNA Maturation and Germ-Line Stem Cell Maintenance Requires Loquacious, a Double-Stranded RNA-Binding Domain Protein (2005, background): “Thibault and co-workers reported a mutant allele of CG6866, loqs , recovered in a large-scale piggyBac transposon mutagenesis screen of Drosophila [39].”
Negative Regulation of Active Zone Assembly by a Newly Identified SR Protein Kinase (2009, background): “In this forward genetic screen, we have analyzed over 2,000 independent transposon insertion lines, including PiggyBac lines on chromosomes 2 and 3 from the Exelixis collection and an independent collection of P{GAL4} lines [13].”