Polymorphic DNA Markers in Black Cherry (Prunus serotina) Are Identified Using Sequences from Sweet Cherry, Peach, and Sour Cherry
By S. L. Downey, A. Iezzoni. 2000
196 citations recorded by the source corpus.
ADDITIONAL INDEX WORDS. microsatellites, simple sequence repeats, chloroplast sequence ABSTRACT. Black cherry (Prunus serotina Ehrh.) is a common secondary forest species with a wide endemic distribution ranging from Nova Scotia south into Mexico, Ecuador, and Peru. Although planted in the United States for its valued lumber, black cherry is essentially a wild species with small fruit ≈6 to 10 mm in diameter. In contrast, in Mexico and Ecuador, domesticates of this species called Capulin, have much larger (2 to 2.5 cm in diameter) edible fruit. To date, no studies of th e genetic diversity within North American black cherry or the ancestral origin of the Capulin types have been conducted. Simple sequence repeats (SSRs, also termed microsatellites) would be the marker of choice for such genetic diversity studies due to their hypervariability; however, generation of these sequence-based markers is expensive. Therefore, our objective was to determine if markers already identified in other Prunus L. species would be informative in black cherry. The black cherry germplasm screened consisted of selections originating from Michigan, Mexico, and Ecuador. A chloroplast DNA marker, originally generated from sour cherry (P. cerasus L.), amplified three different sized products in black cherry. Four of the eight nuclear SSR markers tested from peach (P. persica L. Batsch (Peach Group)), sour cherry, and sweet cherry ( P. avium L.) also amplified and identified polymorphic markers. Together these four primer pairs resolved 54 putative alleles for the 66 black cherry accessions assayed. Success of the sweet cherry, peach, and sour cherry primers in identification of polymorphic markers in black cherry indicates it should be possible to use these markers for comprehensive molecular genetic studies in black cherry.
Subfields: Cell Biology, Molecular Biology, Plant Science
View Paper: https://www.semanticscholar.org/paper/1222fb5574d0134434b180247a185ae95244cb5c
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Citations in the corpus
- Genetic diversity, structure and fruit trait associations in Greek sweet cherry cultivars using microsatellite based (SSR/ISSR) and morpho-physiological markers (2011, methodology): “A comparison of genetic similarity matrices revealed that estimates based on SSRs and ISSRs”
- Development of a second-generation genetic linkage map for peach [Prunus persica (L.) Batsch] and characterization of morphological traits affecting flower and fruit (2006, background): “P. avium PS Genomic library Joobeur et al. 2000; Cantini et al. 2001 5 2 0 PceGA Downey and Iezzoni 2000 4 4 0 PMS Cantini et al. 2001 6 5 1 UCD-CH Struss et al. 2003 10 7 2 EMPA Clarke and Tobutt 2003 20 17 3 EMPaS Vaughan and Russell 2004 9 9 3 Total 54 44 (81.5”
- DNA Fingerprinting of Tetraploid Cherry Germplasm Using Simple Sequence Repeats (2001, background): “…SSR primer pairs from sweet cherry (P. avium), peach [P. persica (Peach group)] (Cipriani et al., 1999; Sosinski et al., 2000), and sour cherry (Downey and Iezzoni, 2000) have been developed; however, the utility of these primers for fingerprinting P. cerasus and P. fruticosa germplasm has not…”
- Cultivar identification and genetic fingerprinting of temperate fruit tree species using DNA markers (2002, methodology): “Thus, Downey and Iezzoni (2000) used one primer pair developed in sweet cherry, and 2 developed in sour cherry and peach to fingerprint 66 black cherry (Prunus serotina) genotypes; Cantini et al. (2001) used 10 primer pairs from peach, sweet and sour cherry to identify 59 tetraploid sour cherry…”
- Analysis of Sweet Cherry (Prunus avium L.) Cultivars Using SSR and AFLP Markers (2003, methodology): “Microsatellites have been recognized as a good source of genetic markers in cherry (Cantini et al., 2001; Downey and Iezzoni, 2000), but a major limitation of SSRs is the time and cost 9394-MolB 904 9/29/03, 10:19:38 AM 905J. AMER.”