An Expanded Palette of Genetically Encoded Ca2+ Indicators

By Yongxin Zhao, Satoko Araki, Jiahui Wu, Takayuki Teramoto, Yu-Fen Chang, Masahiro Nakano, A. Abdelfattah, Manabi Fujiwara, T. Ishihara, T. Nagai and 1 more. Science, 2011

1,272 citations recorded by the source corpus.

Subfields: Biophysics, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/11172f16fd70800c0cdd060f2fe8cc6f31e60a33

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Citations in the corpus

A large field of view two-photon mesoscope with subcellular resolution for in vivo imaging (2016, methodology): “For example, it would be possible to image the primary somatosensory cortex, the primary motor cortex and parts of secondary motor cortex nearly simultaneously (Figure 1A).”
Imaging Neural Activity Using Thy1-GCaMP Transgenic mice (2012, methodology): “2c and Thy1-GCaMP3 transgenic mice were generated by injection of gel-purified DNA into fertilized oocytes using standard techniques (Feng et al., 2004; Zhao et al., 2011a).”
Closed-loop and activity-guided optogenetic control. (2015, positive): “…more suitable when combined with bluelight-sensitive opsins (Inoue et al., 2015; Akerboom et al., 2013; Wu et al., 2013, 2014; Hochbaum et al., 2014; Zhao et al., 2011), as these will more effectively limit opsin-sensor spectral overlap and allow a large range of optogenetic actuators to be used.”
Analysis of functional neuronal connectivity in the Drosophila brain. (2012, background): “The recent development of red-shifted optogenetic controls (Yizhar et al. 2011) and Ca2 sensors (Zhao et al. 2011) may ultimately circumvent this problem, but these newly developed tools have not yet been successfully introduced to Drosophila.”
Optimization of a GCaMP calcium indicator for neural activity imaging (2012, methodology): “A recent report describes variants of GCaMP3, termed “GGECOs”(Zhao et al., 2011), optimized by selecting for maximum F/F in E. coli colonies expressing random mutagenic libraries.”
Genetically encoded calcium indicators for multi-color neural activity imaging and combination with optogenetics (2013, methodology): “…2013 | Volume 6 | Article 2 | 20 The crystal structure of R-GECO1 reveals that the relative orientation of the mApple and CaM domains is significantly shifted relative to that in GCaMP, and thus relative to the homology model used to first rationalize the R-GECO1 mutations (Zhao et al., 2011a).”
Brain-Machine Interfaces: From Basic Science to Neuroprostheses and Neurorehabilitation. (2017, background): “Optical imaging is based on voltage-sensitive (322, 323, 621, 793) and calcium-sensitive (320, 748, 771) fluorescent dyes, and genetically encoded calcium indicators (516, 523, 892) whose signals are monitored using video recordings.”