Droplet vitrification of apical meristems: a cryopreservation protocol applicable to all Musaceae

By B. Panis, Bart Piette, R. Swennen. 2005

312 citations recorded by the source corpus.

Subfields: Molecular Biology, Plant Science

View Paper: https://www.semanticscholar.org/paper/10f5d073a05636c5948540212729e6a8d220f5e5

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Citations in the corpus

Cryopreservation of Phytodiversity: A Critical Appraisal of Theory & Practice (2008, background): “…small (µL) volumes of water containing cryoprotective additives, are deposited on highly efficient, heat-conducting materials such as aluminium foil (Kartha et al., 1982; Schäfer-Menuhr et al., 1996, 1997; Panis et al., 2005) or hypodermic needles (Grout and Henshaw, 1978; Benson et al., 1989).”
Molecular and cytological characterization of the global Musa germplasm collection provides insights into the treasure of banana diversity (2017, background): “…is usually conserved as in vitro proliferating meristems under limited growth conditions (Van den houwe et al. 1995) complemented with cryopreservation to minimize the risk of contamination or human error during subculturing, as well as to avoid somaclonal variation (Panis et al. 2005; Panis 2009).”
Potato Shoot Tip Cryopreservation. A Review (2011, methodology): “In 2004, this protocol was further optimised, and CIP (in cooperation with the Laboratory of Tropical Crop Improvement, Katholieke Universiteit Leuven, Belgium) adopted the droplet vitrification method (see below) initially designed for cryopreserving banana shoot tips also for potato (Gonzalez-Arna”
Biotechnology and Conservation of Plant Biodiversity (2013, background): “This significantly increases the probability of obtaining a vitrified state during cooling and of avoiding devitrification during warming [104].”
Ex Situ and In Situ Conservation of Agricultural Biodiversity: Major Advances and Research Needs (2010, background): “Such tools, including thermal (Differential Scanning Calorimetry (DSC)), biochemical (sugars, lipids, proteins) and histo-cytological analyses, have led to the development of cryo-protocols for conserving more than 200 plant species, including Musa, coffee, and citrus (Engelmann and Takagi, 2000; En”
Genetic Improvement of Banana (2009, unknown): “2004) and proliferating meristems (Panis et al. 2005).”
Status of cryopreservation technologies in plants (crops and forest trees). (2005, methodology): “Leuven, Belgium, 306 banana accessions are currently safely stored using the droplet vitrification method [44], representing more than 1/4 of the world banana collection (unpublished).”
Current Issues in Plant Cryopreservation (2012, background): “dehydration sensitive) seeds that are not storable by any other means, or preservation of specific cultivars of vegetatively propagated crop plants like banana or potato, or for unique ornamental genotypes (Halmagyi et al., 2004; Kaczmarczyk et al., 2011a; Panis et al., 2005).”
Development and large scale application of cryopreservation techniques for shoot and somatic embryo cultures of tropical crops (2007, methodology): “This protocol significantly increases the probability of obtaining a vitrified state during freezing, and of avoiding devitrification during warming (Panis et al. 2005).”
Challenges and Prospects for the Conservation of Crop Genetic Resources in Field Genebanks, in In Vitro Collections and/or in Liquid Nitrogen (2020, background): “Since its first report in 2005 (See Box 2 [77]), this cryopreservation protocols has now been successfully applied to 111 plant species according the publications reported in Web of Science (Situation 1 July 2020) among which some very important staple foods are found, such as potato [96–98], taro [”