Ligand-induced endocytosis of the pattern recognition receptor FLS2 in Arabidopsis.

By S. Robatzek, D. Chinchilla, T. Boller. Genes & Development, 2006

774 citations recorded by the source corpus.

Pattern-recognition receptors (PRRs) trigger innate immune responses in animals and plants. One such PRR is the flagellin receptor FLS2 in Arabidopsis. Here, we demonstrate that a functional fusion of FLS2 to the green fluorescent protein (GFP) resides in cell membranes of most tissues. Stimulation with the flagellin epitope flg22 induces its transfer into intracellular mobile vesicles, followed by degradation. FLS2 internalization depends on cytoskeleton and proteasome functions, and receptor activation. A variant FLS2 mutated in Thr 867, a potential phosphorylation site, binds flg22 normally, but is impaired in flg22 responses and FLS2 endocytosis. We propose that plant cells regulate pathogen-associated molecular pattern (PAMP)-mediated PRR activities by subcellular compartmentalization.

Subfields: Plant Science

View Paper: https://www.semanticscholar.org/paper/103aee2073d0a5e50342ac4b7046733a326521b0

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In Plant Science

1. V. Pastor
2. B. Mauch-Mani
3. V. Flors
4. M. Höfte
5. Estrella Luna
6. J. Ton
7. D. Vleesschauwer
8. Cyril Zipfel

Citations in the corpus

• Quantitative Phosphoproteomics of Early Elicitor Signaling in Arabidopsis*S (2007, background): “Recognition involves the receptor-like kinase FLS2 and activates a downstream response that includes the production of reactive oxygen species, ethylene biosynthesis, activation of a MAPK cascade, and activation of defense gene expression (2–4).”
• A domain swap approach reveals a role of the plant wall-associated kinase 1 (WAK1) as a receptor of oligogalacturonides (2010, background): “Western blot analysis using a GFP-specific polyclonal antibody revealed a specific band corresponding to a molecular mass of approximately 200 kDa for all of the four constructs (41).”
• Plant pattern recognition receptor complexes at the plasma membrane. (2012, background): “Given that FLS2 is endocytosed after flg22 perception [69], it will be interesting to test if PUB12/13-mediated ubiquitination is required for FLS2 internalization.”
• Rhizobacteria-induced systemic resistance (2009, background): “FLS2 is normally present on the plasma membrane and was found to be internalized upon flg22 stimulation (Robatzek et al., 2006).”
• Callose deposition: a multifaceted plant defense response. (2011, background): “188 / Molecular Plant-Microbe Interactions expressed in Arabidopsis roots (Robatzek et al. 2006), the differential callose response to Flg22 and chitosan can only be explained by dissimilarities in the downstream signaling pathways under our growth conditions.”