High-density mapping of single-molecule trajectories with photoactivated localization microscopy

By Suliana Manley, J. Gillette, Georges H. Patterson, Hari Shroff, H. Hess, Eric Betzig, Jennifer Lippincott-Schwartz. Nature Methods, 2008

1,165 citations recorded by the source corpus.

Subfields: Atomic and Molecular Physics, and Optics, Biophysics

View Paper: https://www.semanticscholar.org/paper/102813f3544fd503b69edb285dd995c00903e5a0

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In Atomic and Molecular Physics, and Optics

In Biophysics

Citations in the corpus

Extracting intracellular diffusive states and transition rates from single-molecule tracking data (2013, methodology): “When analyzing the combined data from both Hfq experi ments on untreated cells (23,756 trajectories), we obtained suf ficient evidence to split the intermediate state into two states ( Supplementary Note 1 ).”
Live-cell photoactivated localization microscopy of nanoscale adhesion dynamics (2008, background): “Thus, although individual single-molecule frames were captured fast enough to visualize molecular dynamics at the 20-Hz level, as in previous PALM-based diffusion studie”
Ultrasound Localization Microscopy and Super-Resolution: A State of the Art (2018, neutral): “nanometers, such as adhesion dynamics [80], single-molecule trajectories [81], or microtubules organization [82], are now”
Fluorescent probes for super-resolution imaging in living cells (2008, background): “Recently, PALM and FPALM have been extended to live-cell imagin”
Aging renewal theory and application to random walks (2013, background): “Such dynamics was observed for the motion of lipids in phospholipid membranes [55], single protein molecules in the cell nucleus [56], H-Ras on plasma membranes [57], and of membrane proteins [58].”
Robust single particle tracking in live cell time-lapse sequences (2008, background): “, analysis of such samples does not allow probing of the interactions between particle”
Photoactivatable mCherry for high-resolution two-color fluorescence microscopy (2009, neutral): “2 ) with rsCherry and rsCherryRev, as well as with tdEosFP, which has been used and characterized in previous PALM studie”
Accuracy and precision in quantitative fluorescence microscopy (2009, background): “Although the size of the object in the image will be inaccurate, the centroid of a high SNR image of the object can be used to locate the object with nanometer precision, far beyond the resolution limit (Churchman et al., 2005; Yildiz and Selvin, 2005; Huang et al., 2008; Manley et al., 2008).”