The use of differential scanning fluorimetry to detect ligand interactions that promote protein stability

By F. Niesen, H. Berglund, M. Vedadi. Nature Protocols, 2007

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Structural Basis of RNA Recognition and Activation by Innate Immune Receptor RIG-I (2011, methodology): “5), 50 mM NaCl, 5 mM MgCl 2 , 5 mM DTT and a 5× dilution of SYPRO Orange dye (Invitrogen) as describe”
Meclofenamic acid selectively inhibits FTO demethylation of m6A over ALKBH5 (2014, background): “Although no linear correlation of the degree of temperature shift upon ligand binding with binding affinity could be established (54,55), this high degree of Tm shift of FTO by MA, at least in this one instance, suggests direct binding of the MA inhibitor to the target FTO.”
Direct activation of STING in the tumor microenvironment leads to potent and systemic tumor regression and immunity (2015, background): “DSF measures the stability of complex formation as a function of temperature as an indirect readout of protein-ligand association (Cavlar et al., 2013; Niesen et al., 2007).”
Ligand–receptor interaction platforms and their applications for drug discovery (2012, background): “The unfolding of proteins increases the hydrophobicity of protein molecules, thus increasing fluorescence signal [111].”