TomoJ: tomography software for three-dimensional reconstruction in transmission electron microscopy

By Cédric Messaoudi, Thomas Boudier, Carlos Oscar Sánchez Sorzano, Sergio Marco. BMC Bioinformatics, 2007

300 citations recorded by the source corpus.

BackgroundTransmission electron tomography is an increasingly common three-dimensional electron microscopy approach that can provide new insights into the structure of subcellular components. Transmission electron tomography fills the gap between high resolution structural methods (X-ray diffraction or nuclear magnetic resonance) and optical microscopy. We developed new software for transmission electron tomography, TomoJ. TomoJ is a plug-in for the now standard image analysis and processing software for optical microscopy, ImageJ.ResultsTomoJ provides a user-friendly interface for alignment, reconstruction, and combination of multiple tomographic volumes and includes the most recent algorithms for volume reconstructions used in three-dimensional electron microscopy (the algebraic reconstruction technique and simultaneous iterative reconstruction technique) as well as the commonly used approach of weighted back-projection.ConclusionThe software presented in this work is specifically designed for electron tomography. It has been written in Java as a plug-in for ImageJ and is distributed as freeware.

Subfields: Radiation, Structural Biology, Surfaces, Coatings and Films

View Paper: https://www.semanticscholar.org/paper/0d28b0ca98fb12b022a896f22251b898f25930a3

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In Structural Biology

  1. Johannes E. Schindelin
  2. Benjamin Schmid
  3. Albert Cardona
  4. Jan Sijbers
  5. Willem Jan Palenstijn
  6. Mark Longair
  7. Kees Joost Batenburg
  8. Kevin W. Eliceiri

In Surfaces, Coatings and Films

  1. Jan Sijbers
  2. Benjamin Schmid
  3. Albert Cardona
  4. Willem Jan Palenstijn
  5. Johannes E. Schindelin
  6. Mark Longair
  7. Kees Joost Batenburg

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