Direct reprogramming of human neural stem cells by OCT4
By J. Kim, B. Greber, M. Araúzo-Bravo, Johann Meyer, K. Park, H. Zaehres, Hans Robert Schöler. Nature, 2009
735 citations recorded by the source corpus.
Induced pluripotent stem (iPS) cells have been generated from mouse and human somatic cells by ectopic expression of four transcription factors (OCT4 (also called POU5F1), SOX2, c-Myc and KLF4). We previously reported that Oct4 alone is sufficient to reprogram directly adult mouse neural stem cells to iPS cells. Here we report the generation of one-factor human iPS cells from human fetal neural stem cells (one-factor (1F) human NiPS cells) by ectopic expression of OCT4 alone. One-factor human NiPS cells resemble human embryonic stem cells in global gene expression profiles, epigenetic status, as well as pluripotency in vitro and in vivo. These findings demonstrate that the transcription factor OCT4 is sufficient to reprogram human neural stem cells to pluripotency. One-factor iPS cell generation will advance the field further towards understanding reprogramming and generating patient-specific pluripotent stem cells.
Subfields: Molecular Biology
View Paper: https://www.semanticscholar.org/paper/0c3f8745ddccd4665b3d06535a1dc594dbff461e
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Citations in the corpus
- Epigenetic memory and preferential lineage-specific differentiation in induced pluripotent stem cells derived from human pancreatic islet beta cells. (2011, neutral): “, 2008), and neural stem cells (Kim et al., 2009).”
- Production of De Novo Cardiomyocytes: Human Pluripotent Stem Cell Differentiation and Direct Reprogramming (2012, neutral): “It has been well documented that certain cell types, such as neural stem cells (Kim et al., 2009), can be more easily reprogrammed, but whether this tendency is due to amoremalleable epigenetic state, the existing expression of a subset of pluripotency-associated genes, or the activity of…”
- Induced pluripotency: history, mechanisms, and applications. (2010, background): “Consistent with this notion, somatic cells that express other reprogramming factors endogenously do not require administration of the corresponding exogenous factor(s), as has been demonstrated for neural stem cells that can be converted into iPSCs by Oct4 alone (Table 2; JB Kim et al. 2009a).”
- Generation of induced pluripotent stem cells from human terminally differentiated circulating T cells. (2010, background): “However, recent studies have shown that other human somatic stem cells can be used (Aasen et al., 2008; Eminli et al., 2009; Kim et al., 2009; Ye et al., 2009).”