Regulated chromosomal DNA replication in the absence of a nucleus.
By Johannes C. Walter, Li Sun, J. Newport. 1998
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Citations in the corpus
- The Xenopus Xmus101 protein is required for the recruitment of Cdc45 to origins of DNA replication (2002, methodology): “NPE was prepared as described (Walter et al., 1998).”
- Extracts for Analysis of DNA Replication in a Nucleus-Free System. (2019, methodology): “DISCUSSION The extracts made using these protocols allow for in vitro nucleus-free replication of fully chromatinized DNA templates including small plasmids, which is outlined in Figure 1A (Walter et al. 1998; Arias and Walter 2004).”
- Functional uncoupling of MCM helicase and DNA polymerase activities activates the ATR-dependent checkpoint. (2005, methodology): “In this study, we used a cell-free extract system derived from Xenopus eggs (Walter et al. 1998) to examine the mechanism by which ssDNA accumulates following DNA damage.”
- Initiation of eukaryotic DNA replication: origin unwinding and sequential chromatin association of Cdc45, RPA, and DNA polymerase alpha. (2000, methodology): “Topology of pBluescript Undergoing Replication in NPErestricted to a single round (Walter et al., 1998).”
- Continued primer synthesis at stalled replication forks contributes to checkpoint activation (2010, methodology): “First, we examined the replication of plasmid DNA, which occurs upon sequential incubation of a plasmid with high speed extract (HSE) and nucleoplasmic extract (NPE; Walter et al., 1998; Walter and Newport, 2000).”
- Regulation of the Rev1–pol ζ complex during bypass of a DNA interstrand cross‐link (2015, unknown): “Xenopus egg extracts, DNA replication, and repair assays Preparation of Xenopus egg extracts (HSS and NPE) and DNA replication reactions were performed as described previously (Walter et al , 1998).”
- Nuclear lamins: building blocks of nuclear architecture. (2002, background): “This could establish conditions necessary for achieving high concentrations of these factors (Walter et al. 1998).”