Caveolin-1 Expression Inhibits Wnt/β-Catenin/Lef-1 Signaling by Recruiting β-Catenin to Caveolae Membrane Domains*
By F. Galbiati, D. Volonté, ANTHONY M.C. Brown, D. Weinstein, A. Ben-Ze'ev, R. Pestell, M. Lisanti. Journal of Biological Chemistry, 2000
168 citations recorded by the source corpus.
Caveolin-1 is a principal component of caveolae membranes. In NIH 3T3 cells, caveolin-1 expression is dramatically up-regulated in confluent cells and localizes at areas of cell-cell contact. However, it remains unknown whether caveolin-1 is involved in cell-cell signaling. Here, we examine the potential role of caveolin-1 in regulating β-catenin signaling. β-Catenin plays a dual role in the cell, linking E-cadherin to the actin cytoskeleton and in Wnt signaling by forming a complex with members of the lymphoid enhancing factor (Lef-1) family of transcription factors. We show that E-cadherin, β-catenin, and γ-catenin (plakoglobin) are all concentrated in caveolae membranes. Moreover, we demonstrate that activation of β-catenin/Lef-1 signaling by Wnt-1 or by overexpression of β-catenin itself is inhibited by caveolin-1 expression. We also show that recombinant expression of caveolin-1 in caveolin-1 negative cells is sufficient to recruit β-catenin to caveolae membranes, thereby blocking β-catenin-mediated transactivation. These results suggest that caveolin-1 expression can modulate Wnt/β-catenin/Lef-1 signaling by regulating the intracellular localization of β-catenin.
Subfields: Cell Biology, Molecular Biology
View Paper: https://www.semanticscholar.org/paper/0aa43389d94401c7f58627521601918b48238376
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Citations in the corpus
- Caveolae: From Cell Biology to Animal Physiology (2002, background): “The P104L and TFT mutant Cav-3 proteins act in a dominant-negative fashion by hetero-oligomerizing with wild-type Cav-3 and directing their proteasomal degradation (Galbiati et al., 2000b).”
- Interplay of cadherin-mediated cell adhesion and canonical Wnt signaling. (2010, background): “Caveolin-1 negatively affects canonical Wnt signaling through the recruitment of b-catenin to caveolar membranes (Galbiati et al. 2000).”