Real-time quantitative PCR assay for measurement of avian telomeres

By F. Criscuolo, Pierre Bize, L. Nasir, Neil B. Metcalfe, Christopher Foote, Kate Griffiths, E. Gault, P. Monaghan. 2009

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Oxidative status and telomere length in a long-lived bird facing a costly reproductive event (2011, background): “ferent bird species: the zebra finch and the Alpine swift (Criscuolo et al. 2009).”
Telomere length, non-breeding habitat and return rate in male American redstarts (2013, methodology): “Briefly, genomic DNA was extracted from previously frozen red blood cells using DNeasy Blood and Tissue kit (Qiagen) and following the manufacturer’s protocol.”
Increased brood size leads to persistent eroded telomeres (2014, methodology): “Telomere length was assessed by the quantitative real-time amplification (qPCR) procedure (Cawthon, 2002), previously used in zebra finches (Criscuolo et al., 2009).”
Telomere length in early life predicts lifespan (2012, methodology): “001) between telomere measurements of the same zebra finch samples by the TRF and quantitative PCR methods (37).”
Estimation of census and effective population sizes: the increasing usefulness of DNA-based approaches (2010, methodology): “This telomere length QPCR method will likely need to be calibrated separately for each new species or taxon per- haps using different PCR primers or primer concentra- tions (Criscuolo et al. 2009).”