Oxidative status and telomere length in a long-lived bird facing a costly reproductive event

By Michaël Beaulieu, Sophie Reichert, Y. Maho, A. Ancel, F. Criscuolo. 2011

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Telomere length, non-breeding habitat and return rate in male American redstarts (2013, methodology): “Third, we selected the GAPDH gene as our reference gene (‘single-copy gene’) because it has already been successfully used for telomere measurements by qPCR in birds and, more specifically, in passerine species (Bize et al. 2009; Criscuolo et al. 2009; Beaulieu et al. 2011; Heidinger et al. 2012).”
Increased brood size leads to persistent eroded telomeres (2014, methodology): “We investigated oxidative stress in plasma samples (stored at −80◦C until analysis), with the d-ROMs and the OXY-ADSORBENT tests (Diacron International, Grosseto, Italy) as previously described in birds (Costantini et al., 2007, 2008; Beaulieu et al., 2010, 2011) and mammals (Stier et al., 2012).”
Telomere length in early life predicts lifespan (2012, background): “In support of this possible effect of being in a reproductive state, we found that greater breeding effort did not increase telomere loss, which is similar to the result reported in Adelie penguins (30).”