Inaugural Article: From the Cover: Fast live simultaneous multiwavelength four-dimensional optical microscopy

By P. Carlton, Jérôme Boulanger, Charles Kervrann, Jean-Baptiste Sibarita, Jean Salamero, Susannah Gordon-Messer, D. Bressan, J. Haber, S. Haase, L. Shao and 8 more. 2010

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Deep learning massively accelerates super-resolution localization microscopy (2018, background): “…dyes with rapid switching kinetics, high-power lasers, and fast cameras allows minimization of exposure time without decreasing the signal-to-noise ratio 5,6 , but reaching submil-lisecond exposure remains challenging, and intense irradiation exacerbates phototoxicity in live-cell imaging 7,8 .”
Quantum metrology and its application in biology (2014, background): “ll remains alive, optical damage can permanently disrupt cellular function. Some studies nd that cells cannot divide after being studied, even if they are not visually damaged and continue to respire [12]. In addition to damage, incident light can also heavily disrupt cellular function. Altogether, ”
Highly multiplexed immunofluorescence imaging of human tissues and tumors using t-CyCIF and conventional optical microscopes (2018, methodology): “22 mm 0.21 µm GE OMX Blaze Structured Illumination Microscope 60X 1.42 NA 0.08 x 0.08 mm 0.11 µm 1 1031 1032 properties and thickness of sample, alignment and quality of the optical components in the light path.”