The Fluorescent Toolbox for Assessing Protein Location and Function

By B. Giepmans, S. Adams, Mark Ellisman, Roger Tsien. Science, 2006

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A guide to super-resolution fluorescence microscopy (2010, background): “Using highly specific fluorescent labeling techniques such as immunocytochemistry, in situ hybridization, or fluorescent protein tags, the spatial distribution and dynamics of virtually every subcellular structure, protein, or genomic sequence of interest can be analyzed in chemically fixed or livin”
Identification of a chemical probe for NAADP by virtual screening (2009, background): “The history of fluorescent molecules for probing biology is rich and successfu”
An engineered protein tag for multiprotein labeling in living cells. (2008, background): “Although autofluorescent proteins already provide a straightforward solution to this problem ( Giepmans et al., 2006 ), chemical labeling methods are attractive complements because they allow, for instance, the visualization of proteins in organisms that are not suitable for the expression of autofl”