The growing impact of lyophilized cell-free protein expression systems

By J. P. Hunt, Seung Ook Yang, Kristen M. Wilding, Bradley C. Bundy. 2017

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ABSTRACT Recently reported shelf-stable, on-demand protein synthesis platforms are enabling new possibilities in biotherapeutics, biosensing, biocatalysis, and high throughput protein expression. Lyophilized cell-free protein expression systems not only overcome cold-storage limitations, but also enable stockpiling for on-demand synthesis and completely sterilize the protein synthesis platform. Recently reported high-yield synthesis of cytotoxic protein Onconase from lyophilized E. coli extract preparations demonstrates the utility of lyophilized cell-free protein expression and its potential for creating on-demand biotherapeutics, vaccines, biosensors, biocatalysts, and high throughput protein synthesis.

Subfields: Biotechnology, Molecular Biology, Radiology, Nuclear Medicine and Imaging