Size determination of (bio)conjugated water-soluble colloidal nanoparticles : A comparison of different techniques

By R. Sperling, Tim Liedl, S. Duhr, S. Kudera, M. Zanella, Cheng-An J Lin, W. Chang, D. Braun, Wolfgang J. Parak. 2007

167 citations recorded by the source corpus.

Subfields: Electrochemistry, Electronic, Optical and Magnetic Materials, Physical and Theoretical Chemistry

View Paper: https://www.semanticscholar.org/paper/0158cb2d66c9d2fe8ab1240188e501337ee216b2

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Electronic, Optical and Magnetic Materials

1. Reisa A. Sperling
2. Wolfgang J. Parak
3. G. Ulrich Nienhaus

In Physical and Theoretical Chemistry

1. P. Baaske
2. S. Duhr
3. D. Braun

Citations in the corpus

• Techniques for physicochemical characterization of nanomaterials (2013, background): “By locally heating the sample to generate a temperature gradient, thermophoresis monitors the motion of the sample to evaluate its size and surface potential (Sapsford et al., 2011; Sperling et al., 2007).”
• Surface modification, functionalization and bioconjugation of colloidal inorganic nanoparticles (2010, background): “…and molecular weight of the employed PEG molecules yields thicker shells that can be found to be of the order of a few to tens of nanometers (Sperling et al. 2007), the thickness of about the order of the hydrodynamic diameter of a free PEG molecule forming a random coil (Fee & Alstine…”
• Protein-binding assays in biological liquids using microscale thermophoresis. (2010, background): “Switching off the infrared-laser leads to a re-establishment of the initial homogeneous concentration profile by ordinary diffusion, providing information about the diffusion coefficient of the molecule”
• Surface Functionalization of Nanoparticles with Polyethylene Glycol: Effects on Protein Adsorption and Cellular Uptake. (2015, background): “stretched, but coil, fold, or twist on the NP surface, in full agreement with previous reports.(49) In fact, the size increase with PEG weight rather scales with the radius of gyration of free PEG in its random coil conformation (see Supporting Information for details).”
• Molecular Interaction Studies Using Microscale Thermophoresis (2011, methodology): “In principle, this process is similar to Fluorescence Recovery After Photobleaching (FRAP) experiments(27) and allows one to measure the hydrodynamic radius of molecules.(28) Backdiffusion provides also important information on the reliability of an experiment.”
• The effect of sedimentation and diffusion on cellular uptake of gold nanoparticles (2011, unknown): “In these cases, back diffusion was also not significan”
• Synthesis, characterization, and bioconjugation of fluorescent gold nanoclusters toward biological labeling applications. (2009, methodology): “Hereby similar negative charge on the reference Au NPs and the Au NCs reported in this study was assumed.(46) These data suggest very small effective particle diameters in colloidal solution well below 5 nm.”