MICROSATELLITE AMPLIFICATION FROM MUSEUM FEATHER SAMPLES: EFFECTS OF FRAGMENT SIZE AND TEMPLATE CONCENTRATION ON GENOTYPING ERRORS

By K. Sefc, R. B. Payne, M. Sorenson. 2003

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Noninvasive population genetics: a review of sample source, diet, fragment length and microsatellite motif effects on amplification success and genotyping error rates (2006, background): “As the risk of ADO also increases with amplicon’s length, using longer fragments may therefore in some cases result in a drastic decrease of overall genotyping success (see also Sefc et al. 2003; Buchan et al. 2005).”
Effects of time and rainfall on PCR success using DNA extracted from deer fecal pellets (2010, background): “Problems associated with fecal DNA include contamination by microorganisms or digested food items, sensitivity to seasonal weather, high PCR-inhibitor to DNA ratios, and relatively high amplification and genotyping errors (Buchan et al. 2005; Maudet et al. 2004; Murphy et al. 2003; Sefc et al. 2003”