Essential genes of a minimal bacterium.

By John I. Glass, N. Assad-Garcia, Nina Alperovich, Shibu Yooseph, M. Lewis, M. Maruf, C. Hutchison, Hamilton O. Smith, J. Venter. Proceedings of the National Academy of Sciences of the United States of America, 2006

920 citations recorded by the source corpus.

Subfields: Ecology, Microbiology, Plant Science

View Paper: https://www.semanticscholar.org/paper/010b53fe4a724da49574d89bf9e2f8fc9437ce3a

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Ecology

1. Maureen A. O’Malley
2. J. Dupré

In Microbiology

1. Maureen A. O’Malley
2. J. Dupré

In Plant Science

1. Maureen A. O’Malley
2. J. Dupré

Citations in the corpus

• Fast automated reconstruction of genome-scale metabolic models for microbial species and communities (2018, methodology): “The experimental data used for validation was obtained from (29,37,40–42).”
• A Whole-Cell Computational Model Predicts Phenotype from Genotype (2012, positive): “Bacterial Culture M. genitalium wild-type and mutant strains with single-gene disruptions by transposon insertion (Glass et al., 2006) were grown in Spiroplasma SP-4 culture media at 37 C and 5% CO 2 .”
• DEG 5.0, a database of essential genes in both prokaryotes and eukaryotes (2008, background): “Therefore, to gain the proof of gene dispensability in M. genitalium , Venter’s group isolated and characterized every Tn4001 insertion mutants that were present in individual colonies picked from agar plates (18).”
• Varieties of Living Things: Life at the Intersection of Lineage and Metabolism (2009, background): “They have lost large numbers of their genes and are considered to have ‘little adaptive capability’ (Glass et al. 2006, 425).”
• DEG 10, an update of the database of essential genes that includes both protein-coding genes and noncoding genomic elements (2013, methodology): “Mycobacterium tuberculosis 774 Tn-seq Yes (24) Mycobacterium tuberculosis 742 35 Tn-seq Yes (21) Mycoplasma genitalium 382 Transposon mutagenesis followed by Sanger sequencing, Single-gene knockout Yes (12,59)”