Inhibition of Fertilization and Larval Metamorphosis of the Coral Acropora millepora (Ehrenberg, 1834) by Petroleum Products

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Plasticity of larval pre-competency in response to temperature: observations on multiple broadcast spawning coral species (2010, methodology): “(12:12 h light:dark cycle) using methods described in Negri and Heyward (2000). Each day larvae (10–15 per well) were transferred from the cultures into six-well cell culture plates (n = 3 wells) containing 10 ml FSW.”
Sexual production of corals for reef restoration in the Anthropocene (2020, background): “A range of toxicants, including metals, pesticides, petroleum products, and other industrial products, have been shown to impact larval settlement (Negri & Heyward 2000, Reichelt-Brushett & Harrison 2000, Negri et al. 2005, Lam et al. 2015, Hudspith et al. 2017), and the physical blocking of cues…”
Effects of suspended sediments, dissolved inorganic nutrients and salinity on fertilisation and embryo development in the coral Acropora millepora (Ehrenberg, 1834) (2008, methodology): “The released gametes were collected following the methods of Negri and Heyward (2000).”
Insecticides and a fungicide affect multiple coral life stages (2007, methodology): “The samples stripped into FSW were homogenised and then centrifuged at 1600 × g for 5 min to remove the symbionts.”