A basin‐scale application of environmental DNA assessment for rare endemic species and closely related exotic species in rivers: a case study of giant salamanders in Japan

By S.-I. Fukumoto, A. Ushimaru, Toshifumi Minamoto. 2015

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Methods for the extraction, storage, amplification and sequencing of DNA from environmental samples (2018, result): “Others have similarly reported that the analysis of environmental DNA is a superior approach compared to traditional survey methods for assessing the presence of invasive aquatic animals such as the bluegill sunfish (Takahara & Minimoto 2013) and the Chinese giant salamander (Andrias davidianus; Fuk”
A simple method for preserving environmental DNA in water samples at ambient temperature by addition of cationic surfactant (2017, background): “…of aquatic vertebrates by environmental DNA (eDNA) analysis is currently experiencing rapid expansion in multiple disciplines, such as rare species conservation (Rees et al. 2014; Fukumoto et al. 2015) and invasive species management (Ficetola et al. 2008; Jerde et al. 2011;Takahara et al. 2013).”