Identification of multi-trait PGPR isolates and evaluating their potential as inoculants for wheat

By Anuj Rana, Prof. (Dr.) Baljeet Singh Saharan, Monica Joshi, R. Prasanna, Kanika Kumar, L. Nain. Annals of Microbiology, 2011

221 citations recorded by the source corpus.

Development of an effective plant growth promoting rhizobacteria (PGPR) inoculant necessitates the presence of a diverse set of traits that can help its colonization of the rhizosphere and survival under varying environmental conditions. In our investigation, a set of 100 bacterial isolates from the rhizosphere of wheat plants were screened initially on the basis of a seed germination assay; ten bacterial isolates (AW1–AW10) were selected. These isolates were then tested in vitro for specific PGPR traits, such as the production of IAA, siderophore, ammonia, HCN, P solubilization, ACC deaminase activity, acetylene reduction assay and antifungal activity. Of the ten isolates, AW5 was found to be promising for all PGP attributes. An experiment undertaken in the controlled conditions of the National Phytotron Facility revealed the potential of three isolates (AW1, AW5 and AW7) in enhancing the growth parameters of wheat plants. Characterization of these isolates using polyphasic approaches involving both phenotypic and genotypic attributes led to their identification as Bacillus sp. (AW1), Providencia sp. (AW5), and Brevundimonas diminuta (AW7), respectively. These strains could prove effective PGPR inoculants as they possess a number of traits useful for their establishment and proliferation in soil. The genus Providencia is reported for the first time for its PGP potential, using cultural as well as functional attributes to show its suitability as an inoculant for wheat crop.

Subfields: Plant Science

View Paper: https://www.semanticscholar.org/paper/fee9faa93c610d9f4a07094d5514b59bf30f244e

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Citations in the corpus

Pseudomonas-aided zinc application improves the productivity and biofortification of bread wheat (2018, background): “In the present study, improvement in plant growth and grain yield by inoculationwith MN12 might be due to increased production of IAA, phosphate or Zn solubilisation, and phytosiderophore production (Rana et al. 2011; Rehman et al. 2018b).”
Plant growth promoting potentials of Pseudomonas spp. strain OG isolated from marine water (2013, background): “Efficacy of OG as a potent PGPR was at par with other species of Pseudomonas when compared with the reported data.”
Enhancing micronutrient uptake and yield of wheat through bacterial PGPR consortia (2012, methodology): “Enhancement in the uptake of N, P and micronutrients by the bacterial inoculants is supported by the data on PGPR traits of these strains (Rana et al. 2011).”
Biofortification of wheat through inoculation of plant growth promoting rhizobacteria and cyanobacteria (2012, methodology): “The bacterial strain PW5 was isolated from the wheat rhizosphere and characterized by polyphasic approaches [11].”
Prospecting plant growth promoting bacteria and cyanobacteria as options for enrichment of macro- and micronutrients in grains in rice–wheat cropping sequence (2015, methodology): “on the basis of our previous studies (Manjunath et al., 2011; Nain et al., 2010; Prasanna et al., 2013; Prasanna, Joshi et al., 2012; Rana et al., 2011).”