Nitrogen fixation: Nitrogenase genes and gene expression

By Jonathan P. Zehr, P. J. Turner. 2001

219 citations recorded by the source corpus.

Subfields: Molecular Biology, Pollution, Renewable Energy, Sustainability and the Environment

View Paper: https://www.semanticscholar.org/paper/feced8b33a9f89ac2cd4e306b5a7178aaea0a751

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Molecular Biology

1. R. Foster
2. David M. Karl
3. M. Church
4. A. Paytan
5. T. Shiozaki
6. Ken Furuya
7. S. Takeda
8. M. Ijichi

In Pollution

1. David M. Karl
2. C. Mahaffey
3. M. Church
4. R. Foster
5. K. Björkman
6. A. Paytan
7. T. Shiozaki
8. Ken Furuya

In Renewable Energy, Sustainability and the Environment

1. R. Foster
2. David M. Karl
3. M. Church
4. T. Shiozaki
5. A. Paytan
6. S. Takeda
7. Ken Furuya
8. Jonathan P. Zehr

Citations in the corpus

• Basin scale variability of active diazotrophs and nitrogen fixation in the North Pacific, from the tropics to the subarctic Bering Sea (2017, methodology): “, 2015] using DNA and cDNA templates, Pure Taq Ready-To-Go PCR Beads (GE Healthcare), and a nested PCR protocol [Zehr and Turner, 2001].”
• Seasonality of N2 fixation and nifH gene diversity in the Gulf of Aqaba (Red Sea) (2009, methodology): “In the second round of PCR, the same reaction mixture and conditions were used; however, nifH1 and nifH2 primers (Zehr and Turner 2001) were used and 1 mL (compared to 0.5 mL) of Taq polymerase was added.”
• Regional distributions of nitrogen‐fixing bacteria in the Pacific Ocean (2008, methodology): “To identify diazotroph population diversity, a ,360-base-pair fragment of the nifH gene was amplified by reverse-transcriptase PCR (RT-PCR) from planktonic RNA following the nested PCR approach described in Zehr and Turner (2001).”
• Influence of the Amazon River plume on distributions of free‐living and symbiotic cyanobacteria in the western tropical north Atlantic Ocean (2007, methodology): “In the second round of PCR the same reaction mixture and conditions were used, however, nifH1 and nifH2 primers (Zehr and Turner 2001a) were used and 1 mL of round 1 product was added.”
• Vertical distributions of nitrogen-fixing phylotypes at Stn ALOHA in the oligotrophic North Pacific Ocean (2005, methodology): “To assess the diversity of nifH-containing plankton at Stn ALOHA, samples from 9 depths in the upper 200 m were PCR-amplified using degenerate nifH primers (Zehr & Turner 2001, Steward et al. 2004).”
• Nitrogen fixation by unicellular diazotrophic cyanobacteria in the temperate oligotrophic North Pacific Ocean (2007, methodology): “The six DNA extracts from the 15N2 uptake incubation experiments and one from the DNA depth profiles (25 Oct 25, 12:00 h) were processed using a nested PCR method to amplify a 359 base pair (bp) fragment of the nifH gene following the protocols described in Zehr and Turner (2001).”
• Heterotrophic bacteria as major nitrogen fixers in the euphotic zone of the Indian Ocean (2014, methodology): “Sequencing and Phylogenetic Analysis of the nifH Gene Partial nifH fragments were amplified from samples taken from the 25% light depth, at which nitrogen fixation occurred at all sampling stations, by a nested PCR approach as previously reported [Zehr and Turner, 2001] with a slight modification.”
• Occurrence of benthic microbial nitrogen fixation coupled to sulfate reduction in the seasonally hypoxic Eckernförde Bay, Baltic Sea (2012, background): “…(nmol SO42- cm-3 d-1) 1 100 10000 3.4 nifH gene analysis In general, thenifH gene sequences detected in September samples clustered with those from proteobacteria and those from Cluster III, as defined by Zehr and Turner (2001), while there were no cyanobacterialnifH gene sequences detected (Fig.”