Profiling enzyme activities in vivo using click chemistry methods.

By A. Speers, B. Cravatt. Chemistry and Biology, 2004

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A chemical method for fast and sensitive detection of DNA synthesis in vivo (2008, background): “Although we were able to detect AdUlabeled DNA in cells by fluorescence microscopy, the background was, for unknown reasons, much higher than in the case of EdU (data not shown), as reported for other instances in which click reactions were performed on cells metabolically labeled with azides (12).”
Selective chemical labeling reveals the genome-wide distribution of 5-hydroxymethylcytosine (2010, methodology): “With an azide group present, a biotin tag or any other tag can be installed using Huisgen cycloaddition (click) chemistry for a variety of enrichment, detection and sequencing application”
Click chemistry reactions in medicinal chemistry: Applications of the 1,3‐dipolar cycloaddition between azides and alkynes (2008, methodology): “This technique has been successfully used, for example, to compare the activity of various enzymes in breast cancer cells.(70,71) Figure 16.”