Ligninolytic enzyme production and dye decolorization by Trametes trogii: application of the Plackett–Burman experimental design to evaluate nutritional requirements

By L. Levin, F. Forchiassin, A. Viale. 2005

128 citations recorded by the source corpus.

Abstract The effects of medium components on laccase, manganese peroxidase and glyoxal oxidase production by Trametes trogii (MYA 28-11) have been studied using Plackett–Burman (PB) experimental design. Copper had the highest positive influence on ligninolytic enzyme production. T. trogii extracellular fluids obtained in the medium which rendered the highest ligninolytic production (45.32 U/ml laccase, 214.5 mU/ml manganese peroxidase and 116 mU/ml glyoxal oxidase) also showed the greatest ability to decolorize the dyes Ponceau 2R (a xylidine derivative), malachite green and anthraquinone blue (at rates of 2.14, 1.35 μg and 3 mg dye/(ml h), respectively). The relationship among decolorization rates and ligninolytic enzyme activities was analyzed by multiple regression. The results fit a linear plus interactions model. The comparison of the response surfaces obtained, suggests that while laccase activity has a greater importance in xylidine degradation, manganese peroxidase activity plays the major role in malachite green decolorization.

Subfields: Biotechnology, Plant Science

View Paper: https://www.semanticscholar.org/paper/fdb3f338e16dc851d7dae652ba52e8937ca0b455

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Citations in the corpus

Treatment of Colored Effluents with Lignin-Degrading Enzymes: An Emerging Role of Marine-Derived Fungi (2008, methodology): “In recent years, several statistical designs collectively under response surface methodology have been introduced into the fermentation field to replace the “one-factor-at-a-time” method (Levin et al. 2005).”
Laccase: A Review of Its Past and Its Future in Bioremediation (2011, background): “Levin et al. (2005) found that extracellular fluids obtained growing T. trogii MYA 28–11 with the highest ligninolytic enzyme production (45 units/ml laccase, 0.21 units/ml manganese peroxidase, and 0.12 units/ml glyoxal oxidase) displayed the greatest ability to decolorize the dyes Ponceau 2R (a…”
Screening for Ligninolytic Enzyme Production by Diverse Fungi from Tunisia (2005, background): “Levin et al. (2005) reported that optimal conditions for Lac production by Trametes trogii are 28 � C, pH 4–6.5 and 10–20 g l )1 of”