PCR Primers That Amplify Fungal rRNA Genes from Environmental Samples

By J. Borneman, R. J. Hartin. Applied and Environmental Microbiology, 2000

637 citations recorded by the source corpus.

Subfields: Cell Biology, Molecular Biology, Plant Science

View Paper: https://www.semanticscholar.org/paper/fd14432181e0bf19e872e791b6279015f103d803

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Citations in the corpus

Extracellular DNA in soil and sediment: fate and ecological relevance (2009, background): “The release of DNA from fungal cells has been less investigated than that of DNA from bacterial cells despite 70% of the DNA molecules in soil being of fungal origin (Smit et al. 1999; Borneman and Hartin 2000; van Elsas et al. 2000).”
Response of soil microbial communities to compost amendments (2006, methodology): “18S rRNA gene was amplified by PCR using the primer nuSSU-0817-5 0 (TTAGCATGGAATAATRRAATAGGA) and the primer nu-SSU-1536-3 0(ATTGCAATGCYCTATCCCCA) (Borneman and Hartin, 2000) fluorescently labeled with the dye D3 (Beckman Coulter).”
Chemical and Biological Gradients along the Damma Glacier Soil Chronosequence, Switzerland (2011, positive): “For specific amplification of fungal 18S rRNA, the primers nu-SSU0817 (FAM-labeled) and nu-SSU1536 were used (Borneman and Hartin, 2000).”
Common and distinguishing features of the bacterial and fungal communities in biological soil crusts and shrub root zone soils (2014, methodology): “For determination of fungal 18S rRNA gene copy numbers, amplificationwas performed using primers nu-SSU1196F and nu-SSU-1536R (Borneman and Hartin, 2000).”
Thematic Issue on Horizontal Gene Transfer Review Release and persistence of extracellular DNA in the environment (2007, positive): “It has been estimated that up to 70% of the DNA molecules present in soil is of fungal origin (Borneman and Hartin, 2000; Smit et al., 1999; van Elsas et al., 2000).”
Microbial diversity and soil functions (2003, methodology): “The assessment of fungal diversity in soil by molecular techniques has not been as successful as the characterization of bacterial diversity because the concentration of fungal DNA is much less than that of bacterial DNA (Borneman & Hartin, 2000).”
Fungal diversity in wounded stems of Aquilaria malaccensis (2010, background): “A large variety of primers currently exist for the amplification of different regions of fungal DNA (White et al. 1990; Smit et al. 1999; Borneman and Hartin 2000).”
Molecular and functional diversity in soil micro-organisms (2002, background): “This approach, therefore, provides a means of studying changes in fungal communities in soil with the potential for identification by sequencing bands of interest from TGGE or DGGE gels.”
Soil microbial diversity and activity linked to crop yield and quality in a dryland organic wheat production system (2016, background): “, 1993); and for fungal rRNA genes were nu-SSU-1196F (50- GGA AAC TCA CCA GGT CCA GA-30) and nu-SSU-1536R (50-ATT GCA ATG CYC TAT CCC CA-30) (Borneman and Hartin, 2000).”