Production of exopolysaccharides by Lactobacillus helveticus MB2-1 and its functional characteristics in vitro
By Wei Li, Juan Ji, X. Rui, Jiaojiao Yu, Weizhi Tang, Xiaohong Chen, Mei Jiang, Mingsheng Dong. 2014
148 citations recorded by the source corpus.
Abstract The production, characterization and functional properties in vitro of exopolysaccharides (EPS) of Lactobacillus helveticus MB2-1 with whey solution as basal medium were investigated. For EPS production, the strain grew well at 37 °C and 3 g/L MgSO 4 with soya peptone as optimum nitrogen source. The maximum yield of EPS (753 mg/L) was obtained with the presence of lactose and soya peptone at concentrations of 80 g/L and 20 g/L, respectively. The EPS was found to be heteropolymer composed of galactose, glucose and mannose in a molar ratio of 1.00:1.69:3.54 with molecular weight about 2.0 × 10 5 Da. The EPS showed as a strong emulsifier in different oils/hydrocarbon substrates. The EPS also showed potent antioxidant effects, such as the abilities to scavenge hydroxyl, superoxide and DPPH radicals, and chelating ability on ferrous ion in a dose-dependent manner. In addition, antibiofilm activities by inhibition of three biofilm forming foodborne pathogenic bacteria were evident. The investigation demonstrated that the EPS from L. helveticus MB2-1 had potentials as a multi-functional natural ingredient for therapeutic and health foods.
Subfields: Food Science, Molecular Biology, Plant Science
View Paper: https://www.semanticscholar.org/paper/fcb964292c6b8da8a5e38f5e73944636417e9f6c
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Citations in the corpus
- Production, characterization and In vitro antioxidant activities of exopolysaccharide from Weissella cibaria GA44 (2018, background): “Li et al. (2014) reported dose-dependent DPPH free radical scavenging activity of crude EPS from L. helveticus MB2-1.”