Vibrational imaging based on stimulated Raman scattering microscopy

By P. Nanakumar, A. Kovalev, A. Volkmer. 2009

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A stimulated Raman scattering microscope with near-infrared picosecond laser pulses at high repetition rates (76 MHz) and radio-frequency lock-in detection is accomplished. Based on stimulated Raman loss detection, we demonstrate noninvasive point-by-point vibrational mapping of chemical and biological samples with high sensitivity and without the requirement for labeling of the sample with natural or artificial fluorophores. We experimentally demonstrate a major benefit of this technique, which is the capability to respond exclusively to the linear Raman-resonance properties of the sample, thus allowing a direct quantitative interpretation of image contrast in terms of the number density of Raman-active modes.

Subfields: Analytical Chemistry, Biophysics, Electronic, Optical and Magnetic Materials

View Paper: https://www.semanticscholar.org/paper/fc6b38a5ecbacc6c5ebe8256cc27d1b70eb1d3c3