Factors affecting transformation efficiency of embryogenic callus of Upland cotton (Gossypium hirsutum) with Agrobacterium tumefaciens

By Shuangxia Jin, Xianlong Zhang, Shaoguang Liang, Y. Nie, Xiao-ping Guo, Chao Huang. Plant Cell Tissue and Organ Culture, 2004

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Identification of a novel elite genotype for in vitro culture and genetic transformation of cotton (2006, unknown): “To verify the presence of introduced npt-II gene in kanamycin-resistant calli, genomic DNA was extracted as our previous report (Jin et al. 2005) and PCR was conducted by using the primers P1: 5’- TCCGGCGCTTGGGTGGAGAG- 3’ and P2: 5’- CTGGCGCGAGCCCCTGATGCT- 3’ for amplifying a 470-bp coding-region…”
Jatropha curcas: a review on biotechnological status and challenges (2011, background): “Combined with genetic engineering, micropropagation through somatic embryogenesis provides an efficient means of producing a large number of elite or transgenic plants (Jin et al. 2005; Li et al. 2006a, b), which has been incorporated into many breeding programmes.”
Regulation of Somatic Embryogenesis in Higher Plants (2010, methodology): “Combined with genetic engineering, micropropagation through somatic embryogenesis provides an efficient means of producing a large number of elite or transgenic plants ( Jin et al , 2005 ; Li et al , 2006 ), which has been incorporated into many breeding programs.”