Estimating accuracy of RNA-Seq and microarrays with proteomics

By Zheng Yan, Ying Xu, C. Menzel, P. Khaitovich. BMC Genomics, 2009

335 citations recorded by the source corpus.

BackgroundMicroarrays revolutionized biological research by enabling gene expression comparisons on a transcriptome-wide scale. Microarrays, however, do not estimate absolute expression level accurately. At present, high throughput sequencing is emerging as an alternative methodology for transcriptome studies. Although free of many limitations imposed by microarray design, its potential to estimate absolute transcript levels is unknown.ResultsIn this study, we evaluate relative accuracy of microarrays and transcriptome sequencing (RNA-Seq) using third methodology: proteomics. We find that RNA-Seq provides a better estimate of absolute expression levels.ConclusionOur result shows that in terms of overall technical performance, RNA-Seq is the technique of choice for studies that require accurate estimation of absolute transcript levels.

Subfields: Molecular Biology

View Paper: https://www.semanticscholar.org/paper/f9ea610049774d42048f026a81d86f5d5270d27a

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In Molecular Biology

Citations in the corpus

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